3D Bioprinted Hydrogel Microfluidic Devices for Parallel Drug Screening

Anant Bhusal, Elvan Dogan, Daniel Nieto, Seyed Ali Mousavi Shaegh, Berivan Cecen, Amir K. Miri

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Conventional high-throughput screening (HTS) platforms suffer from the need for large cell volumes, high reagent consumption, significant assembly cost, and handling efforts. The assembly of three-dimensional (3D) bioprinted hydrogel-based microfluidic chips within platforms can address these problems. We present a continuous and seamless manufacturing approach to create a bioprinted microfluidic chips with a circular pattern scalable toward HTS platforms. Digital light processing 3D bioprinting is used to tune the local permeability of our chip, made of polyethylene glycol diacrylate and cell-laden gelatin methacryloyl, for creating predefined gradients of biochemical properties. We measured the flow-induced physical characteristics, the mass transport of drug agents, and the biological features of the proposed chip. We measured reactive oxygen species from the encapsulated cells through an integrated process and showed the capacity of the hydrogel-based chip for creating drug/agent gradients. This work introduces a chip design based on a hydrogel that can be changed and could be used for modern HTS platforms such as in vitro organoids.

Original languageEnglish (US)
Pages (from-to)4480-4492
Number of pages13
JournalACS Applied Bio Materials
Volume5
Issue number9
DOIs
StatePublished - 2022

All Science Journal Classification (ASJC) codes

  • Biomaterials
  • General Chemistry
  • Biomedical Engineering
  • Biochemistry, medical

Keywords

  • bioprinting
  • digital light processing
  • high-throughput screening
  • hydrogel
  • microfluidics
  • organ-on-a-chip

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