A BODIPY-Based Far-Red-Absorbing Fluorescent Probe for Hypochlorous Acid Imaging

Zhaoxiong Wan, Shupei Yu, Qi Wang, John Tobia, Hao Chen, Zhanjun Li, Xuan Liu, Yuanwei Zhang

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


Hypochlorous acid (HClO) is produced by white blood cells to defend against injury and bacteria. However, as one of the reactive oxygen species, high intracellular HClO concentration could lead to chronic diseases that affect the cardiovascular and nervous systems. To monitor HClO concentrations in bio-samples, the fluorescent probe is preferred to have: a) Absorbability in the far-red window with reduced light toxicity and improved tissue penetration depth; b) Ratiometric features for accurate analysis. In this study, we reported a far-red ratiometric HClO fluorescence probe based on BODIPY chromophore and aldoxime sensing group. Not only the color change of the probe solution can be detected by the naked eye, but also the emission ratios (I645/I670) showed a significant increase upon the introduction of HClO. More importantly, the feasibility of HClO monitoring in biosamples was demonstrated in vitro using a confocal microscope.

Original languageEnglish (US)
Article numbere202100250
Issue number4
StatePublished - Apr 2022

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Physical and Theoretical Chemistry
  • Organic Chemistry


  • cell imaging
  • hypochlorous acid
  • ratiometric sensing
  • reactive oxygen species


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