A BODIPY-Based Water-Soluble Fluorescent Probe for Mitochondria Targeting

Binglin Sui; Simon Tang; Adam W. Woodward; Bosung Kim; Kevin D. Belfield

doi:10.1002/ejoc.201600238

A BODIPY-Based Water-Soluble Fluorescent Probe for Mitochondria Targeting

Binglin Sui, Simon Tang, Adam W. Woodward, Bosung Kim, Kevin D. Belfield

Research output: Contribution to journal › Article › peer-review

39 Scopus citations

Abstract

A water-soluble fluorescent mitochondrial targeting probe is reported. The probe bears a BODIPY-based fluorophore, two triphenylphosphonium (TPP) groups as mitochondrial targeting moieties, and two PEG groups to increase water solubility and enhance biocompatibility. As a mitochondria-selective fluorescent probe, the new BODIPY derivative exhibited many desirable properties, such as water solubility, high fluorescence quantum yield, solvent independent photophysical properties, pH insensitivity, good photostability, low cytotoxicity, and excellent mitochondrial targeting ability. Fluorescence microscopy colocalization experiments demonstrated the ability of this new probe to target mitochondria within living cells for bioimaging.

Original language	English (US)
Pages (from-to)	2851-2857
Number of pages	7
Journal	European Journal of Organic Chemistry
Volume	2016
Issue number	16
DOIs	https://doi.org/10.1002/ejoc.201600238
State	Published - Jun 2016

All Science Journal Classification (ASJC) codes

Physical and Theoretical Chemistry
Organic Chemistry

Keywords

Cytotoxicity
Fluorescent probes
Imaging agents
Mitochondria targeting
Photophysics

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10.1002/ejoc.201600238

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title = "A BODIPY-Based Water-Soluble Fluorescent Probe for Mitochondria Targeting",

abstract = "A water-soluble fluorescent mitochondrial targeting probe is reported. The probe bears a BODIPY-based fluorophore, two triphenylphosphonium (TPP) groups as mitochondrial targeting moieties, and two PEG groups to increase water solubility and enhance biocompatibility. As a mitochondria-selective fluorescent probe, the new BODIPY derivative exhibited many desirable properties, such as water solubility, high fluorescence quantum yield, solvent independent photophysical properties, pH insensitivity, good photostability, low cytotoxicity, and excellent mitochondrial targeting ability. Fluorescence microscopy colocalization experiments demonstrated the ability of this new probe to target mitochondria within living cells for bioimaging.",

keywords = "Cytotoxicity, Fluorescent probes, Imaging agents, Mitochondria targeting, Photophysics",

author = "Binglin Sui and Simon Tang and Woodward, {Adam W.} and Bosung Kim and Belfield, {Kevin D.}",

note = "Publisher Copyright: {\textcopyright} 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim",

year = "2016",

month = jun,

doi = "10.1002/ejoc.201600238",

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T1 - A BODIPY-Based Water-Soluble Fluorescent Probe for Mitochondria Targeting

AU - Sui, Binglin

AU - Tang, Simon

AU - Woodward, Adam W.

AU - Kim, Bosung

AU - Belfield, Kevin D.

PY - 2016/6

Y1 - 2016/6

N2 - A water-soluble fluorescent mitochondrial targeting probe is reported. The probe bears a BODIPY-based fluorophore, two triphenylphosphonium (TPP) groups as mitochondrial targeting moieties, and two PEG groups to increase water solubility and enhance biocompatibility. As a mitochondria-selective fluorescent probe, the new BODIPY derivative exhibited many desirable properties, such as water solubility, high fluorescence quantum yield, solvent independent photophysical properties, pH insensitivity, good photostability, low cytotoxicity, and excellent mitochondrial targeting ability. Fluorescence microscopy colocalization experiments demonstrated the ability of this new probe to target mitochondria within living cells for bioimaging.

AB - A water-soluble fluorescent mitochondrial targeting probe is reported. The probe bears a BODIPY-based fluorophore, two triphenylphosphonium (TPP) groups as mitochondrial targeting moieties, and two PEG groups to increase water solubility and enhance biocompatibility. As a mitochondria-selective fluorescent probe, the new BODIPY derivative exhibited many desirable properties, such as water solubility, high fluorescence quantum yield, solvent independent photophysical properties, pH insensitivity, good photostability, low cytotoxicity, and excellent mitochondrial targeting ability. Fluorescence microscopy colocalization experiments demonstrated the ability of this new probe to target mitochondria within living cells for bioimaging.

KW - Cytotoxicity

KW - Fluorescent probes

KW - Imaging agents

KW - Mitochondria targeting

KW - Photophysics

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DO - 10.1002/ejoc.201600238

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JO - European Journal of Organic Chemistry

JF - European Journal of Organic Chemistry

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ER -

A BODIPY-Based Water-Soluble Fluorescent Probe for Mitochondria Targeting

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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