A new water-soluble near-neutral ratiometric fluorescent pH indicator

Sheng Yao; Katherine J. Schafer-Hales; Kevin D. Belfield

doi:10.1021/ol7026366

A new water-soluble near-neutral ratiometric fluorescent pH indicator

Sheng Yao, Katherine J. Schafer-Hales, Kevin D. Belfield

Research output: Contribution to journal › Article › peer-review

121 Scopus citations

Abstract

Donor-π-acceptor fluorene derivative 1c is a near-neutral pH indicator whose pK_a of ∼7.0 was determined by both absorption and fluorescence methods. 1c satisfies important criteria for a sensitive ratiomeric fluorescent pH indicator with a distinctive isoemissive point, good dispersion in cell cytosol, and low cytotoxicity. Furthermore, its 2PA cross section of 100 GM in its neutral form suggests its potential in two-photon fluorescence imaging applications.

Original language	English (US)
Pages (from-to)	5645-5648
Number of pages	4
Journal	Organic Letters
Volume	9
Issue number	26
DOIs	https://doi.org/10.1021/ol7026366
State	Published - Dec 20 2007
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biochemistry
Physical and Theoretical Chemistry
Organic Chemistry

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10.1021/ol7026366

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abstract = "Donor-π-acceptor fluorene derivative 1c is a near-neutral pH indicator whose pKa of ∼7.0 was determined by both absorption and fluorescence methods. 1c satisfies important criteria for a sensitive ratiomeric fluorescent pH indicator with a distinctive isoemissive point, good dispersion in cell cytosol, and low cytotoxicity. Furthermore, its 2PA cross section of 100 GM in its neutral form suggests its potential in two-photon fluorescence imaging applications.",

author = "Sheng Yao and Schafer-Hales, {Katherine J.} and Belfield, {Kevin D.}",

year = "2007",

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AU - Belfield, Kevin D.

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AB - Donor-π-acceptor fluorene derivative 1c is a near-neutral pH indicator whose pKa of ∼7.0 was determined by both absorption and fluorescence methods. 1c satisfies important criteria for a sensitive ratiomeric fluorescent pH indicator with a distinctive isoemissive point, good dispersion in cell cytosol, and low cytotoxicity. Furthermore, its 2PA cross section of 100 GM in its neutral form suggests its potential in two-photon fluorescence imaging applications.

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A new water-soluble near-neutral ratiometric fluorescent pH indicator

Abstract

All Science Journal Classification (ASJC) codes

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