A new water-soluble near-neutral ratiometric fluorescent pH indicator

Sheng Yao, Katherine J. Schafer-Hales, Kevin D. Belfield

Research output: Contribution to journalArticlepeer-review

119 Scopus citations


Donor-π-acceptor fluorene derivative 1c is a near-neutral pH indicator whose pKa of ∼7.0 was determined by both absorption and fluorescence methods. 1c satisfies important criteria for a sensitive ratiomeric fluorescent pH indicator with a distinctive isoemissive point, good dispersion in cell cytosol, and low cytotoxicity. Furthermore, its 2PA cross section of 100 GM in its neutral form suggests its potential in two-photon fluorescence imaging applications.

Original languageEnglish (US)
Pages (from-to)5645-5648
Number of pages4
JournalOrganic Letters
Issue number26
StatePublished - Dec 20 2007
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Physical and Theoretical Chemistry
  • Organic Chemistry


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