A new water-soluble near-neutral ratiometric fluorescent ph indicator

Sheng Yao, Katherine J. Schafer-Hales, Kevin D. Belfield

Research output: Contribution to conferencePaperpeer-review


Donor-π-acceptor fluorene derivative 1 is a near-neutral pH indicator whose pKa of ~7.0 was determined by both absorption and fluorescence methods. 1 satisfies important criteria for a sensitive ratiomeric fluorescent pH indicator with a distinctive isoemissive point, good dispersion in cell cytosol, and low cytotoxicity. Furthermore, its 2PA cross-section of 100 GM in its neutral form suggests its potential in two-photon fluorescence imaging applications.

Original languageEnglish (US)
StatePublished - 2008
Externally publishedYes
EventBiomedical Optics, BIOMED 2008 - St. Petersburg, FL, United States
Duration: Mar 16 2008Mar 19 2008


OtherBiomedical Optics, BIOMED 2008
Country/TerritoryUnited States
CitySt. Petersburg, FL

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering
  • Biomaterials
  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics


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