A rapid field-use assay for mismatch number and location of hybridized DNAs

I. Fang Cheng, Satyajyoti Senapati, Xinguang Cheng, Sagnik Basuray, Hsien Chang Chang, Hsueh Chia Chang

Research output: Contribution to journalArticlepeer-review

62 Scopus citations


Molecular dielectrophoresis (DEP) is employed to rapidly (<ms) trap ssDNA molecules in a flowing solution to a cusp-shaped nanocolloid assembly on a chip with a locally amplified AC electric field gradient. By tuning AC field frequency and DNA DEP mobility relative to its electrophoretic mobility due to electrostatic repulsion from like-charged nanocolloids, mismatch-specific binding of DNA molecules at the cusp is achieved by the converging flow, with a concentration factor about 6 orders of magnitude higher than the bulk, thus allowing fluorescent quantification of concentrated DNAs at the singularity in a generic buffer, at room temperature within a minute. Optimum flow rate and the corresponding hybridization rate change by nearly a factor of 2 with a single mismatch in the 26 base docking sequence and are also sensitive to the mismatch location. This dielectrophoresis and shear enhanced pico-molar sensitivity and SNP selectivity can hence be used for field-use DNA detection/identification.

Original languageEnglish (US)
Pages (from-to)828-831
Number of pages4
JournalLab on a Chip
Issue number7
StatePublished - 2010
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biochemistry
  • General Chemistry
  • Biomedical Engineering


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