A receptor-based bioassay for quantitative detection of gallium

H. Xu; E. Lee; O. A. Sadik; R. Bakhtiar; J. Drader; C. Hendrikson

doi:10.1021/ac990902m

A receptor-based bioassay for quantitative detection of gallium

H. Xu, E. Lee, O. A. Sadik, R. Bakhtiar, J. Drader, C. Hendrikson

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5 Scopus citations

Abstract

The detection of gallium in biological samples is required due to its role in the diagnosis of tumor and for possible treatment of malignancies. However, the use of purely instrumental techniques is unsuitable for detection of low levels of gallium in biological matrixes. We have synthesized new protein conjugates based on 4-(2-pyridylazo) ligands. The conjugates were successfully employed for the detection of gallium in biological matrixes using a nonantibody-based sandwich assay format. The recovery level obtained was between 97 and 101.3 with a relative standard deviation of less than 5%. The assay resulted in a detection limit of 5 x 10^-8 M and a remarkable selectivity for gallium(III) relative to other metals investigated. The new method provided adequate accuracy for gallium applicable for animal physiology and clinical toxicology.

Original language	English (US)
Pages (from-to)	5271-5278
Number of pages	8
Journal	Analytical Chemistry
Volume	71
Issue number	23
DOIs	https://doi.org/10.1021/ac990902m
State	Published - Dec 1 1999
Externally published	Yes

All Science Journal Classification (ASJC) codes

Analytical Chemistry

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10.1021/ac990902m

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title = "A receptor-based bioassay for quantitative detection of gallium",

abstract = "The detection of gallium in biological samples is required due to its role in the diagnosis of tumor and for possible treatment of malignancies. However, the use of purely instrumental techniques is unsuitable for detection of low levels of gallium in biological matrixes. We have synthesized new protein conjugates based on 4-(2-pyridylazo) ligands. The conjugates were successfully employed for the detection of gallium in biological matrixes using a nonantibody-based sandwich assay format. The recovery level obtained was between 97 and 101.3 with a relative standard deviation of less than 5%. The assay resulted in a detection limit of 5 x 10-8 M and a remarkable selectivity for gallium(III) relative to other metals investigated. The new method provided adequate accuracy for gallium applicable for animal physiology and clinical toxicology.",

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year = "1999",

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T1 - A receptor-based bioassay for quantitative detection of gallium

AU - Xu, H.

AU - Lee, E.

AU - Sadik, O. A.

AU - Bakhtiar, R.

AU - Drader, J.

AU - Hendrikson, C.

PY - 1999/12/1

Y1 - 1999/12/1

N2 - The detection of gallium in biological samples is required due to its role in the diagnosis of tumor and for possible treatment of malignancies. However, the use of purely instrumental techniques is unsuitable for detection of low levels of gallium in biological matrixes. We have synthesized new protein conjugates based on 4-(2-pyridylazo) ligands. The conjugates were successfully employed for the detection of gallium in biological matrixes using a nonantibody-based sandwich assay format. The recovery level obtained was between 97 and 101.3 with a relative standard deviation of less than 5%. The assay resulted in a detection limit of 5 x 10-8 M and a remarkable selectivity for gallium(III) relative to other metals investigated. The new method provided adequate accuracy for gallium applicable for animal physiology and clinical toxicology.

AB - The detection of gallium in biological samples is required due to its role in the diagnosis of tumor and for possible treatment of malignancies. However, the use of purely instrumental techniques is unsuitable for detection of low levels of gallium in biological matrixes. We have synthesized new protein conjugates based on 4-(2-pyridylazo) ligands. The conjugates were successfully employed for the detection of gallium in biological matrixes using a nonantibody-based sandwich assay format. The recovery level obtained was between 97 and 101.3 with a relative standard deviation of less than 5%. The assay resulted in a detection limit of 5 x 10-8 M and a remarkable selectivity for gallium(III) relative to other metals investigated. The new method provided adequate accuracy for gallium applicable for animal physiology and clinical toxicology.

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JF - Analytical Chemistry

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A receptor-based bioassay for quantitative detection of gallium

Abstract

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