A receptor-based bioassay for quantitative detection of gallium

H. Xu, E. Lee, O. A. Sadik, R. Bakhtiar, J. Drader, C. Hendrikson

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


The detection of gallium in biological samples is required due to its role in the diagnosis of tumor and for possible treatment of malignancies. However, the use of purely instrumental techniques is unsuitable for detection of low levels of gallium in biological matrixes. We have synthesized new protein conjugates based on 4-(2-pyridylazo) ligands. The conjugates were successfully employed for the detection of gallium in biological matrixes using a nonantibody-based sandwich assay format. The recovery level obtained was between 97 and 101.3 with a relative standard deviation of less than 5%. The assay resulted in a detection limit of 5 x 10-8 M and a remarkable selectivity for gallium(III) relative to other metals investigated. The new method provided adequate accuracy for gallium applicable for animal physiology and clinical toxicology.

Original languageEnglish (US)
Pages (from-to)5271-5278
Number of pages8
JournalAnalytical Chemistry
Issue number23
StatePublished - Dec 1 1999
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry


Dive into the research topics of 'A receptor-based bioassay for quantitative detection of gallium'. Together they form a unique fingerprint.

Cite this