TY - JOUR
T1 - A series of fluorene-based two-photon absorbing molecules
T2 - Synthesis, linear and nonlinear characterization, and bioimaging
AU - Andrade, Carolina D.
AU - Yanez, Ciceron O.
AU - Rodriguez, Luis
AU - Belfield, Kevin D.
PY - 2010/6/18
Y1 - 2010/6/18
N2 - The synthesis, structural, and photophysical characterization of a series of new fluorescent donor-acceptor and acceptor-acceptor molecules, based on the fluorenyl ring system, with two-photon absorbing properties is described. These new compounds exhibited large Stokes shifts, high fluorescence quantum yields, and significantly, high two-photon absorption cross sections, making them well suited for two-photon fluorescence microscopy imaging. Confocal and two-photon fluorescence microscopy imaging of COS-7 and HCT 116 cells incubated with probe I showed endosomal selectivity, demonstrating the potential of this class of fluorescent probes in multiphoton fluorescence microscopy.
AB - The synthesis, structural, and photophysical characterization of a series of new fluorescent donor-acceptor and acceptor-acceptor molecules, based on the fluorenyl ring system, with two-photon absorbing properties is described. These new compounds exhibited large Stokes shifts, high fluorescence quantum yields, and significantly, high two-photon absorption cross sections, making them well suited for two-photon fluorescence microscopy imaging. Confocal and two-photon fluorescence microscopy imaging of COS-7 and HCT 116 cells incubated with probe I showed endosomal selectivity, demonstrating the potential of this class of fluorescent probes in multiphoton fluorescence microscopy.
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U2 - 10.1021/jo1005075
DO - 10.1021/jo1005075
M3 - Article
C2 - 20481596
AN - SCOPUS:77953511861
SN - 0022-3263
VL - 75
SP - 3975
EP - 3982
JO - Journal of Organic Chemistry
JF - Journal of Organic Chemistry
IS - 12
ER -