A series of fluorene-based two-photon absorbing molecules: Synthesis, linear and nonlinear characterization, and bioimaging

Carolina D. Andrade, Ciceron O. Yanez, Luis Rodriguez, Kevin D. Belfield

Research output: Contribution to journalArticlepeer-review

59 Scopus citations

Abstract

The synthesis, structural, and photophysical characterization of a series of new fluorescent donor-acceptor and acceptor-acceptor molecules, based on the fluorenyl ring system, with two-photon absorbing properties is described. These new compounds exhibited large Stokes shifts, high fluorescence quantum yields, and significantly, high two-photon absorption cross sections, making them well suited for two-photon fluorescence microscopy imaging. Confocal and two-photon fluorescence microscopy imaging of COS-7 and HCT 116 cells incubated with probe I showed endosomal selectivity, demonstrating the potential of this class of fluorescent probes in multiphoton fluorescence microscopy.

Original languageEnglish (US)
Pages (from-to)3975-3982
Number of pages8
JournalJournal of Organic Chemistry
Volume75
Issue number12
DOIs
StatePublished - Jun 18 2010
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Organic Chemistry

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