TY - JOUR
T1 - A water-soluble diaminostilbene derivative as a two-photon fluorescent probe
AU - Yao, Sheng
AU - Schafer-Hales, Katherine J.
AU - Cohanoschi, Ion
AU - Hernández, Florencio E.
AU - Belfield, Kevin
PY - 2006/8/1
Y1 - 2006/8/1
N2 - A hydrophilic trans-4,4′-diaminostilbene derivative was prepared by the introduction of amideamino peripheral substituents. Very high aqueous solubility was achieved (>15 mM), making it possible as a fluorescent probe for biological imaging. The relatively straightforward synthetic methodology has great potential to transform even large hydrophobic two-photon absorbing chromophores into hydrophilic derivatives. The fluorescence quantum yield of 0.27 for the diaminostilbene in water is sufficiently high for use as a fluorescent probe. In addition, two-photon fluorescence images of NT2 cells stained by this probe were also obtained.
AB - A hydrophilic trans-4,4′-diaminostilbene derivative was prepared by the introduction of amideamino peripheral substituents. Very high aqueous solubility was achieved (>15 mM), making it possible as a fluorescent probe for biological imaging. The relatively straightforward synthetic methodology has great potential to transform even large hydrophobic two-photon absorbing chromophores into hydrophilic derivatives. The fluorescence quantum yield of 0.27 for the diaminostilbene in water is sufficiently high for use as a fluorescent probe. In addition, two-photon fluorescence images of NT2 cells stained by this probe were also obtained.
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U2 - 10.1055/s-2006-948165
DO - 10.1055/s-2006-948165
M3 - Article
AN - SCOPUS:33747066727
SN - 0936-5214
SP - 1863
EP - 1866
JO - Synlett
JF - Synlett
IS - 12
ER -