A water-soluble diaminostilbene derivative as a two-photon fluorescent probe

Sheng Yao, Katherine J. Schafer-Hales, Ion Cohanoschi, Florencio E. Hernández, Kevin D. Belfield

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


A hydrophilic trans-4,4′-diaminostilbene derivative was prepared by the introduction of amideamino peripheral substituents. Very high aqueous solubility was achieved (>15 mM), making it possible as a fluorescent probe for biological imaging. The relatively straightforward synthetic methodology has great potential to transform even large hydrophobic two-photon absorbing chromophores into hydrophilic derivatives. The fluorescence quantum yield of 0.27 for the diaminostilbene in water is sufficiently high for use as a fluorescent probe. In addition, two-photon fluorescence images of NT2 cells stained by this probe were also obtained.

Original languageEnglish (US)
Pages (from-to)1863-1866
Number of pages4
Issue number12
StatePublished - Aug 1 2006
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Organic Chemistry


  • Fluorescent probe
  • PAMAM: hydrophilic
  • Two-photon
  • Two-photon microscopy
  • Z-scan


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