An in vitro 3-D model of collagen-based fiber constituents for peripheral nerve conduits

M. L. Siriwardane, K. E. DeRosa, G. L. Collins, B. J. Pfister

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

An in vitro three-dimensional (3D) fiber-reinforced hydrogel system consisting of collagen was used as a peripheral nerve conduit model for evaluating axon growth from sensory neurons and the migration of glial cells. Micron-size collagen fibers created by wet spinning were used as firm guiding wires in a 3D collagen gel to promote a direction for regenerating dorsal root ganglia (DRG) axons and migrating Schwann cells. Type I collagen extracted from rat tail tendons was used to prepare these collagen-based components for an engineered nerve conduit design. Crosslinking treatments with genipin and glutaraldehyde were evaluated to improve mechanical properties, swelling response and thermal behavior to facilitate guided growth and long-term viability of neurons and glial cells for future in vivo models.

Original languageEnglish (US)
Title of host publication2012 38th Annual Northeast Bioengineering Conference, NEBEC 2012
Pages420-421
Number of pages2
DOIs
StatePublished - 2012
Event38th Annual Northeast Bioengineering Conference, NEBEC 2012 - Philadelphia, PA, United States
Duration: Mar 16 2012Mar 18 2012

Publication series

Name2012 38th Annual Northeast Bioengineering Conference, NEBEC 2012

Other

Other38th Annual Northeast Bioengineering Conference, NEBEC 2012
Country/TerritoryUnited States
CityPhiladelphia, PA
Period3/16/123/18/12

All Science Journal Classification (ASJC) codes

  • Bioengineering

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