Analysis of flow cytometric data

J. Quinn, Li Hsin Loo, R. Capocasale, P. Bugelski, M. Kam, L. Hrebien

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Fluorescence Activated Cytometry is a research tool whose potential has yet to be fully exploited. Currently it is used for cluster hunting and cell counting. In this study, we separate cell clusters by identifying local minima in the cell density gradient to identify boundaries between cell types. These clusters were then analyzed for changes in intensity of four parameters marked by fluorescent antibody labels. The results are a statistical analysis of differences in the cell clusters which can be interpreted to understand what changes are occurring in the various cell populations on many levels.

Original languageEnglish (US)
Title of host publicationProceedings of the IEEE 29th Annual Northeast Bioengineering Conference
EditorsStanley Reisman, Richard Foulds, Bruno Mantilla
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages244-245
Number of pages2
ISBN (Electronic)0780377672
DOIs
StatePublished - Jan 1 2003
Externally publishedYes
Event29th IEEE Annual Northeast Bioengineering Conference, NEBC 2003 - Newark, United States
Duration: Mar 22 2003Mar 23 2003

Publication series

NameProceedings of the IEEE Annual Northeast Bioengineering Conference, NEBEC
Volume2003-January
ISSN (Print)1071-121X
ISSN (Electronic)2160-7001

Other

Other29th IEEE Annual Northeast Bioengineering Conference, NEBC 2003
CountryUnited States
CityNewark
Period3/22/033/23/03

All Science Journal Classification (ASJC) codes

  • Bioengineering

Keywords

  • Analysis of variance
  • Bones
  • Data analysis
  • Gaussian distribution
  • Mice
  • Microscopy
  • Particle measurements
  • Probability distribution
  • Strips
  • Testing

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