Antimicrobial and Anticancer Activity of a Novel Peptide (Musterolysin) Extracted from Slurry of Mustard Oil Refinery Industry

Bacterial infections and cancer have been most prevalent diseases throughout the World. The discovery and development of new antibacterial and anticancer agents is critical. If such a valuable medicinal product is created from waste resources from the oil refinery business, academicians and industrialists will pay close attention. The sequential extraction process followed by RP-HPLCfractions was used to purify and identify a new peptide from mustard oil refinery sludge. The sequence of the peptide was determined as NH₂-KYQFFVP-COOH using MALDI TOF/TOF Mass spectrometry. The antibacterial activity of the peptide was determined against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis, and Bacillus subtilis. The proapoptotic activity of the peptide was also determined against Hep2 (Human epidermoid cancer cell line). These findings suggest that readily available bio-waste might be a novel source of bioactive antibacterial and proapoptotic lipopeptides.