Autonomous Multielectrode System for Monitoring the Interactions of Isoflavonoids with Lung Cancer Cells

Silvana Andreescu, Omowunmi A. Sadik, Dennis W. McGee, Shin Ichiro Suye

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53 Scopus citations


The cytotoxic effect of isoflavonoids in the development of different forms of cancer has been reported by epidemiological and dietary studies. Consequently, there is a search for an accurate and reliable method for monitoring the interactions of these chemicals with cancerous cells. We have developed and optimized a fully autonomous electrochemical biosensor for studying the role of isoflavonoids on A549 lung adenocarcinoma cell line. This advanced biosensor uses a prototype 96-electrode (DOX-96) well-type device that allows the measurement of cell respiratory activity via the consumption of dissolved oxygen. The system provides a continuous, real-time monitoring of cell activity upon exposure to naturally occurring polyphenols, specifically resveratrol, genistein, and quercetin. The system is equipped with a multipotentiostat, a 96-electrode well for measurements and cell culturing with 3 disposable electrodes fitted into each well. A comparison with classical "cell culture" techniques indicates that the biosensor provides real-time measurement with no added reagents. A detection limit of 1 × 104 was recorded versus 200 and 6 × 103 cells/well for MTT and fluorescence assays, respectively. This method was optimized with respect to cell stability, reproducibility, applied potential, cell density per well, volume/composition of cell culture medium per well, and incubation. Others include total measuring time, temperature, and sterilization procedure. This study represents a basic research tool that may allow researchers to study the type, level, and specific influence of isoflavonoids on cells.

Original languageEnglish (US)
Pages (from-to)2321-2330
Number of pages10
JournalAnalytical Chemistry
Issue number8
StatePublished - Apr 15 2004
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry


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