Cell encapsulation in gelatin bioink impairs 3D bioprinting resolution

Rachel Schwartz; Matthew Malpica; Gary L. Thompson; Amir K. Miri

doi:10.1016/j.jmbbm.2019.103524

Cell encapsulation in gelatin bioink impairs 3D bioprinting resolution

Rachel Schwartz, Matthew Malpica, Gary L. Thompson, Amir K. Miri

Research output: Contribution to journal › Article › peer-review

55 Scopus citations

Abstract

Recent advances in three-dimensional (3D) bioprinting technologies have enabled precise patterning of cellular components along with biomimetic constructs for tissue engineering and regenerative medicine. The viscoelasticity of bioinks regulate printability and the smallest feature size in 3D bioprinted constructs. The impact of cellular components is typically neglected when choosing 3D bioprinting parameters. In this short communication, we quantified the effect of cell densities on the printability of hydrogel bioinks. Unexpectedly, our results show that encapsulated cells reduced the steady shear viscosity of gelatin-based bioinks by approximately 50% and the minimum force for onset of flow by approximately 30%. These results may justify the lower spatial resolution in 3D bioprinted cell-laden hydrogels.

Original language	English (US)
Article number	103524
Journal	Journal of the Mechanical Behavior of Biomedical Materials
Volume	103
DOIs	https://doi.org/10.1016/j.jmbbm.2019.103524
State	Published - Mar 2020
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biomaterials
Biomedical Engineering
Mechanics of Materials

Keywords

3D bioprinting
Printability
Resolution
Shear-thinning

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10.1016/j.jmbbm.2019.103524

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title = "Cell encapsulation in gelatin bioink impairs 3D bioprinting resolution",

abstract = "Recent advances in three-dimensional (3D) bioprinting technologies have enabled precise patterning of cellular components along with biomimetic constructs for tissue engineering and regenerative medicine. The viscoelasticity of bioinks regulate printability and the smallest feature size in 3D bioprinted constructs. The impact of cellular components is typically neglected when choosing 3D bioprinting parameters. In this short communication, we quantified the effect of cell densities on the printability of hydrogel bioinks. Unexpectedly, our results show that encapsulated cells reduced the steady shear viscosity of gelatin-based bioinks by approximately 50% and the minimum force for onset of flow by approximately 30%. These results may justify the lower spatial resolution in 3D bioprinted cell-laden hydrogels.",

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AU - Schwartz, Rachel

AU - Malpica, Matthew

AU - Thompson, Gary L.

AU - Miri, Amir K.

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AB - Recent advances in three-dimensional (3D) bioprinting technologies have enabled precise patterning of cellular components along with biomimetic constructs for tissue engineering and regenerative medicine. The viscoelasticity of bioinks regulate printability and the smallest feature size in 3D bioprinted constructs. The impact of cellular components is typically neglected when choosing 3D bioprinting parameters. In this short communication, we quantified the effect of cell densities on the printability of hydrogel bioinks. Unexpectedly, our results show that encapsulated cells reduced the steady shear viscosity of gelatin-based bioinks by approximately 50% and the minimum force for onset of flow by approximately 30%. These results may justify the lower spatial resolution in 3D bioprinted cell-laden hydrogels.

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KW - Resolution

KW - Shear-thinning

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Cell encapsulation in gelatin bioink impairs 3D bioprinting resolution

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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