Cell encapsulation in gelatin methacryloyl bioinks impairs microscale diffusion properties

Elvan Dogan, Christina Holshue, Anant Bhusal, Roshni Shukla, Amir K. Miri

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Light-assisted bioprinted gelatin methacryloyl (GelMA) constructs have been used for cell-laden microtissues and organoids. GelMA can be loaded by desired cells, which can regulate the biophysical properties of bioprinted constructs. We study how the degree of methacrylation (MA degree), GelMA mass concentration, and cell density change mass transport properties. We introduce a fluorescent-microscopy-based method of biotransport testing with improved sensitivity compared to the traditional particle tracking methods. The diffusion capacity of GelMA with a higher MA significantly decreased compared to a lower MA. Opposed to a steady range of linear elastic moduli, the diffusion coefficient in GelMA varied when cell densities ranged from 0 to 10 × 106 cells/ml. A comparative study of different cell sizes showed a higher diffusivity coefficient for the case of larger cells. The results of this study can help bioengineers and scientists to better control the biotransport characteristics in light-assisted bioprinted microtissues and organoids.

Original languageEnglish (US)
Article number1193970
JournalFrontiers in Bioengineering and Biotechnology
Volume11
DOIs
StatePublished - 2023

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Histology
  • Biomedical Engineering

Keywords

  • bioink
  • bioprinting
  • diffusivity
  • gelatin methacryloyl
  • mass transport

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