Colorimetric Cu2+ detection using DNA-modified gold-nanoparticle aggregates as probes and click chemistry

Xiaoyang Xu, Weston L. Daniel, Wei Wei, Chad A. Mirkin

Research output: Contribution to journalArticlepeer-review

220 Scopus citations

Abstract

The colorimetric detection of Cu2+ is demonstrated using DNA-modified gold nanoparticle (Au NP) aggregates as probes and a copper-catalyzed click reaction to initiate the cross-coupling of alkynes and azides that terminate the DNA on the particles. The ligation of two oligonucleotides via click chemistry and copper ions raises the melting temperature of that duplex, which can be monitored colorimetrically using the optical properties of the Au NPs.

Original languageEnglish (US)
Pages (from-to)623-626
Number of pages4
JournalSmall
Volume6
Issue number5
DOIs
StatePublished - Mar 8 2010
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biomaterials
  • Chemistry(all)
  • Materials Science(all)

Keywords

  • Click chemistry
  • Colorimetric detection
  • Copper
  • DNA
  • Nanoparticle conjugates

Fingerprint Dive into the research topics of 'Colorimetric Cu<sup>2+</sup> detection using DNA-modified gold-nanoparticle aggregates as probes and click chemistry'. Together they form a unique fingerprint.

Cite this