The colorimetric detection of Cu2+ is demonstrated using DNA-modified gold nanoparticle (Au NP) aggregates as probes and a copper-catalyzed click reaction to initiate the cross-coupling of alkynes and azides that terminate the DNA on the particles. The ligation of two oligonucleotides via click chemistry and copper ions raises the melting temperature of that duplex, which can be monitored colorimetrically using the optical properties of the Au NPs.
All Science Journal Classification (ASJC) codes
- Materials Science(all)
- Click chemistry
- Colorimetric detection
- Nanoparticle conjugates