Colorimetric high-throughput assay for alkene epoxidation catalyzed by cytochrome P450 BM-3 variant 139-3

Keyphrases

• P450 BM3 100%
• Cytochrome P450 (CYP450) 100%
• Alkene Epoxidation 100%
• High-throughput Assay 100%
• Directed Evolution 33%
• Limit of Detection 33%
• Biotechnology 33%
• Fatty Acids 33%
• Alkanes 33%
• Styrene 33%
• Turnover Rate 33%
• Highly Active 33%
• Cell Lysate 33%
• O-nitrobenzyl 33%
• Pyridine 33%
• Sensitivity Threshold 33%
• Nucleophile 33%
• Random mutation 33%
• Hydroxylation 33%
• Styrene Oxidation 33%
• NADPH Depletion 33%
• Small Molecule Libraries 33%
• Styrene Oxide 33%
• Alkenes 33%

Chemistry

• Alkene 100%
• Styrene 100%
• Cytochrome P450 100%
• Epoxidation 100%
• Alkane 50%
• NADPH 50%
• Nucleophile 50%
• Styrene Oxide 50%
• Hydroxylation 50%

Biochemistry, Genetics and Molecular Biology

• Cytochrome P450 100%
• Epoxidation 100%
• Enzyme 50%
• Alpha Oxidation 50%
• Precursor 50%
• Cell Lysate 50%
• Hydroxylation 50%