TY - JOUR
T1 - Competition between two microbial populations in a sequencing fed‐batch reactor
T2 - Theory, experimental verification, and implications for waste treatment applications
AU - Dikshitulu, S.
AU - Baltzis, Basil
AU - Lewandowski, G. A.
AU - Pavlou, S.
PY - 1993/1/1
Y1 - 1993/1/1
N2 - Competition between two microbial populations for a single pollutant (phenol) was studied in a sequencing fed‐batch reactor (SFBR). A mathematical model describing this system was developed and tested experimentally. It is based on specific growth rate expressions revealed from pure culture batch experiments. The species employed were Pseudomonas putida (ATCC 17514) and Pseudomonas resinovorans (ATCC 14235). It was found that both species biodegrade phenol following inhibitory kinetics which can be described by Andrews' expression. The model predicts that the dynamics of a SFBR, and the kinetics of biodegradation, result in a complex set of operating regimes in which neither species, only one species, or both species can survive at steady cycle. The model also predicts the existence of multiple outcomes, achievable from different start‐up conditions, in some domains of the operating parameter space. Experimental results confirmed the model predictions. There was excellent agreement between predicted and measured concentrations of phenol, total biomass, and the biomass of each individual species. This study shows how serious discrepancies can arise in scale‐up of biodegradation data if population dynamics are not taken into account. It also further confirms experimentally the theory of microbial competition in periodically forced bioreactors. © 1993 John Wiley & Sons, Inc.
AB - Competition between two microbial populations for a single pollutant (phenol) was studied in a sequencing fed‐batch reactor (SFBR). A mathematical model describing this system was developed and tested experimentally. It is based on specific growth rate expressions revealed from pure culture batch experiments. The species employed were Pseudomonas putida (ATCC 17514) and Pseudomonas resinovorans (ATCC 14235). It was found that both species biodegrade phenol following inhibitory kinetics which can be described by Andrews' expression. The model predicts that the dynamics of a SFBR, and the kinetics of biodegradation, result in a complex set of operating regimes in which neither species, only one species, or both species can survive at steady cycle. The model also predicts the existence of multiple outcomes, achievable from different start‐up conditions, in some domains of the operating parameter space. Experimental results confirmed the model predictions. There was excellent agreement between predicted and measured concentrations of phenol, total biomass, and the biomass of each individual species. This study shows how serious discrepancies can arise in scale‐up of biodegradation data if population dynamics are not taken into account. It also further confirms experimentally the theory of microbial competition in periodically forced bioreactors. © 1993 John Wiley & Sons, Inc.
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U2 - 10.1002/bit.260420513
DO - 10.1002/bit.260420513
M3 - Article
C2 - 18613087
AN - SCOPUS:0027201602
SN - 0006-3592
VL - 42
SP - 643
EP - 656
JO - Biotechnology and Bioengineering
JF - Biotechnology and Bioengineering
IS - 5
ER -