Computational and empirical trans-hydrogen bond deuterium isotope shifts suggest that N1-N3 A:U hydrogen bonds of RNA are shorter than those of A:T hydrogen bonds of DNA

Yong Ick Kim, Marlon N. Manalo, Lisa M. Peréz, Andy LiWang

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Density functional theory calculations of isolated Watson-Crick A:U and A:T base pairs predict that adenine 13C2 trans-hydrogen bond deuterium isotope shifts due to isotopic substitution at the pyrimidine H3, 2hΔ13C2, are sensitive to the hydrogen-bond distance between the N1 of adenine and the N3 of uracil or thymine, which supports the notion that 2hΔ13C2 is sensitive to hydrogen-bond strength. Calculated 2hΔ13C2 values at a given N1-N3 distance are the same for isolated A:U and A:T base pairs. Replacing uridine residues in RNA with 5-methyl uridine and substituting deoxythymidines in DNA with deoxyuridines do not statistically shift empirical 2hΔ13C2 values. Thus, we show experimentally and computationally that the C7 methyl group of thymine has no measurable affect on 2hΔ13C2 values. Furthermore, 2hΔ13C2 values of modified and unmodified RNA are more negative than those of modified and unmodified DNA, which supports our hypothesis that RNA hydrogen bonds are stronger than those of DNA. It is also shown here that 2hΔ13C2 is context dependent and that this dependence is similar for RNA and DNA.

Original languageEnglish (US)
Pages (from-to)229-236
Number of pages8
JournalJournal of Biomolecular NMR
Volume34
Issue number4
DOIs
StatePublished - Apr 2006
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Spectroscopy

Keywords

  • DNA
  • Density functional theory
  • Deuterium isotope shift
  • Hydrogen bond
  • NMR
  • RNA

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