Construction and characterization of a bacterial artificial chromosome library of marine macroalga Porphyra yezoensis (Rhodophyta)

Yong Deng Dai, Ge Zhao, Song Xuan Jin, Liang Yang Ji, Lin Duan De, Li Weng Man, Bin Wang

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

A large-DNA-fragment library is necessary for research into the Porphyra genome. In this study, a bacterial artificial chromosome (BAC) library of Porphyra yezoensis was constructed and characterized. The library contains 54,144 BAC clones with an average insert size of about 65 kb and fewer than 0.7% of clones without large inserts. Therefore, its capacity is more than 6.6 P. yezoensis genome equivalents, and the probability of recovering any nuclear DNA sequence from the library is higher than 99%. The library shows good fidelity and stability. A putative trehalose-6-phosphate synthase (TPS) gene was successfully screened out from the library. The above results show that the library is useful for gene cloning and genomic research in P. yezoensis.

Original languageEnglish (US)
Pages (from-to)375-386
Number of pages12
JournalPlant Molecular Biology Reporter
Volume22
Issue number4
DOIs
StatePublished - Dec 2004
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Plant Science

Keywords

  • BAC library
  • Gene cloning
  • Genomic research
  • Porphyra yezoensis
  • Trehalose-6-phosphate synthase (TPS) gene

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