Construction and characterization of a bacterial artificial chromosome library of marine macroalga Porphyra yezoensis (Rhodophyta)

Yong Deng Dai; Ge Zhao; Song Xuan Jin; Liang Yang Ji; Lin Duan De; Li Weng Man; Bin Wang

doi:10.1007/bf02772680

Construction and characterization of a bacterial artificial chromosome library of marine macroalga Porphyra yezoensis (Rhodophyta)

Yong Deng Dai, Ge Zhao, Song Xuan Jin, Liang Yang Ji, Lin Duan De, Li Weng Man, Bin Wang

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

A large-DNA-fragment library is necessary for research into the Porphyra genome. In this study, a bacterial artificial chromosome (BAC) library of Porphyra yezoensis was constructed and characterized. The library contains 54,144 BAC clones with an average insert size of about 65 kb and fewer than 0.7% of clones without large inserts. Therefore, its capacity is more than 6.6 P. yezoensis genome equivalents, and the probability of recovering any nuclear DNA sequence from the library is higher than 99%. The library shows good fidelity and stability. A putative trehalose-6-phosphate synthase (TPS) gene was successfully screened out from the library. The above results show that the library is useful for gene cloning and genomic research in P. yezoensis.

Original language	English (US)
Pages (from-to)	375-386
Number of pages	12
Journal	Plant Molecular Biology Reporter
Volume	22
Issue number	4
DOIs	https://doi.org/10.1007/bf02772680
State	Published - Dec 2004
Externally published	Yes

All Science Journal Classification (ASJC) codes

Molecular Biology
Plant Science

Keywords

BAC library
Gene cloning
Genomic research
Porphyra yezoensis
Trehalose-6-phosphate synthase (TPS) gene

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10.1007/bf02772680

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title = "Construction and characterization of a bacterial artificial chromosome library of marine macroalga Porphyra yezoensis (Rhodophyta)",

abstract = "A large-DNA-fragment library is necessary for research into the Porphyra genome. In this study, a bacterial artificial chromosome (BAC) library of Porphyra yezoensis was constructed and characterized. The library contains 54,144 BAC clones with an average insert size of about 65 kb and fewer than 0.7% of clones without large inserts. Therefore, its capacity is more than 6.6 P. yezoensis genome equivalents, and the probability of recovering any nuclear DNA sequence from the library is higher than 99%. The library shows good fidelity and stability. A putative trehalose-6-phosphate synthase (TPS) gene was successfully screened out from the library. The above results show that the library is useful for gene cloning and genomic research in P. yezoensis.",

keywords = "BAC library, Gene cloning, Genomic research, Porphyra yezoensis, Trehalose-6-phosphate synthase (TPS) gene",

author = "Dai, {Yong Deng} and Ge Zhao and Jin, {Song Xuan} and Ji, {Liang Yang} and De, {Lin Duan} and Man, {Li Weng} and Bin Wang",

note = "Funding Information: We thank Dr R. Wing, Clemson University, Clemson, SC, for kindly providing the BAC cloning vector, pCUGIBAC1. This work was supported by the National High Technology Research and Development Program of China (2001AA621090) and supported by the Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences. We thank Dr H. Zalkin (Purdue University, West Lafayette, IN) for help with the manuscript.",

year = "2004",

month = dec,

doi = "10.1007/bf02772680",

language = "English (US)",

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AU - Dai, Yong Deng

AU - Zhao, Ge

AU - Jin, Song Xuan

AU - Ji, Liang Yang

AU - De, Lin Duan

AU - Man, Li Weng

AU - Wang, Bin

N1 - Funding Information: We thank Dr R. Wing, Clemson University, Clemson, SC, for kindly providing the BAC cloning vector, pCUGIBAC1. This work was supported by the National High Technology Research and Development Program of China (2001AA621090) and supported by the Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences. We thank Dr H. Zalkin (Purdue University, West Lafayette, IN) for help with the manuscript.

PY - 2004/12

Y1 - 2004/12

N2 - A large-DNA-fragment library is necessary for research into the Porphyra genome. In this study, a bacterial artificial chromosome (BAC) library of Porphyra yezoensis was constructed and characterized. The library contains 54,144 BAC clones with an average insert size of about 65 kb and fewer than 0.7% of clones without large inserts. Therefore, its capacity is more than 6.6 P. yezoensis genome equivalents, and the probability of recovering any nuclear DNA sequence from the library is higher than 99%. The library shows good fidelity and stability. A putative trehalose-6-phosphate synthase (TPS) gene was successfully screened out from the library. The above results show that the library is useful for gene cloning and genomic research in P. yezoensis.

AB - A large-DNA-fragment library is necessary for research into the Porphyra genome. In this study, a bacterial artificial chromosome (BAC) library of Porphyra yezoensis was constructed and characterized. The library contains 54,144 BAC clones with an average insert size of about 65 kb and fewer than 0.7% of clones without large inserts. Therefore, its capacity is more than 6.6 P. yezoensis genome equivalents, and the probability of recovering any nuclear DNA sequence from the library is higher than 99%. The library shows good fidelity and stability. A putative trehalose-6-phosphate synthase (TPS) gene was successfully screened out from the library. The above results show that the library is useful for gene cloning and genomic research in P. yezoensis.

KW - BAC library

KW - Gene cloning

KW - Genomic research

KW - Porphyra yezoensis

KW - Trehalose-6-phosphate synthase (TPS) gene

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Construction and characterization of a bacterial artificial chromosome library of marine macroalga Porphyra yezoensis (Rhodophyta)

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All Science Journal Classification (ASJC) codes

Keywords

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