Construction and characterization of a bacterial artificial chromosome library of maize inbred line 77Ht2

Jiliang Yang; Qinghua Wang; Daiyong Deng; Manli Weng; Xiaoyan Yin; Demin Jin; Juren Zhang; Bin Wang

doi:10.1007/BF02774242

Construction and characterization of a bacterial artificial chromosome library of maize inbred line 77Ht2

Jiliang Yang, Qinghua Wang, Daiyong Deng, Manli Weng, Xiaoyan Yin, Demin Jin, Juren Zhang, Bin Wang

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Maize inbred line 77Ht2 contains agriculturally important genes and has been widely used in corn breeding in China. A bacterial artificial chromosome (BAC) library of 77Ht2 has been constructed in order to identify useful genes and to facilitate the study of the maize genome. The library contains 175104 clones with an average insert size of 57 kb and represents about 4 maize haploid genome equivalents. Characterization of the library showed less than 0.5% of clones to not contain large inserts. Significant contamination of chloroplast and mitochondria DNA was not detected. BAC clones (152 arrays) were stored in 96 microtiter plates, with each well containing 12 clones. This is the first maize BAC library constructed in China. It is well suited for map-based cloning of maize genes and genome physical mapping.

Original language	English (US)
Pages (from-to)	159-169
Number of pages	11
Journal	Plant Molecular Biology Reporter
Volume	21
Issue number	2
DOIs	https://doi.org/10.1007/BF02774242
State	Published - Jun 2003
Externally published	Yes

All Science Journal Classification (ASJC) codes

Molecular Biology
Plant Science

Keywords

77Ht2
Bacterial artificial chromosome
Maize

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10.1007/BF02774242

Cite this

@article{6fe47f0201b64354a0a1999e5b3161ec,

title = "Construction and characterization of a bacterial artificial chromosome library of maize inbred line 77Ht2",

abstract = "Maize inbred line 77Ht2 contains agriculturally important genes and has been widely used in corn breeding in China. A bacterial artificial chromosome (BAC) library of 77Ht2 has been constructed in order to identify useful genes and to facilitate the study of the maize genome. The library contains 175104 clones with an average insert size of 57 kb and represents about 4 maize haploid genome equivalents. Characterization of the library showed less than 0.5% of clones to not contain large inserts. Significant contamination of chloroplast and mitochondria DNA was not detected. BAC clones (152 arrays) were stored in 96 microtiter plates, with each well containing 12 clones. This is the first maize BAC library constructed in China. It is well suited for map-based cloning of maize genes and genome physical mapping.",

keywords = "77Ht2, Bacterial artificial chromosome, Maize",

author = "Jiliang Yang and Qinghua Wang and Daiyong Deng and Manli Weng and Xiaoyan Yin and Demin Jin and Juren Zhang and Bin Wang",

note = "Funding Information: This library is a global resource and is available to all scientists upon written request. This research was supported by the Specific Project of Plant Gene Transformation Project (J00-A-002) and the State High Technology Project 2001AA211111. We thank Dr Yang Dian'er, Dr Wang Yueguang, Dr Xing Quanhua, Dr Liang Fengshan, Dr Liang Chunyang, Mr Li Jun,",

year = "2003",

month = jun,

doi = "10.1007/BF02774242",

language = "English (US)",

volume = "21",

pages = "159--169",

journal = "Plant Molecular Biology Reporter",

issn = "0735-9640",

publisher = "Springer New York",

number = "2",

}

TY - JOUR

T1 - Construction and characterization of a bacterial artificial chromosome library of maize inbred line 77Ht2

AU - Yang, Jiliang

AU - Wang, Qinghua

AU - Deng, Daiyong

AU - Weng, Manli

AU - Yin, Xiaoyan

AU - Jin, Demin

AU - Zhang, Juren

AU - Wang, Bin

N1 - Funding Information: This library is a global resource and is available to all scientists upon written request. This research was supported by the Specific Project of Plant Gene Transformation Project (J00-A-002) and the State High Technology Project 2001AA211111. We thank Dr Yang Dian'er, Dr Wang Yueguang, Dr Xing Quanhua, Dr Liang Fengshan, Dr Liang Chunyang, Mr Li Jun,

PY - 2003/6

Y1 - 2003/6

N2 - Maize inbred line 77Ht2 contains agriculturally important genes and has been widely used in corn breeding in China. A bacterial artificial chromosome (BAC) library of 77Ht2 has been constructed in order to identify useful genes and to facilitate the study of the maize genome. The library contains 175104 clones with an average insert size of 57 kb and represents about 4 maize haploid genome equivalents. Characterization of the library showed less than 0.5% of clones to not contain large inserts. Significant contamination of chloroplast and mitochondria DNA was not detected. BAC clones (152 arrays) were stored in 96 microtiter plates, with each well containing 12 clones. This is the first maize BAC library constructed in China. It is well suited for map-based cloning of maize genes and genome physical mapping.

AB - Maize inbred line 77Ht2 contains agriculturally important genes and has been widely used in corn breeding in China. A bacterial artificial chromosome (BAC) library of 77Ht2 has been constructed in order to identify useful genes and to facilitate the study of the maize genome. The library contains 175104 clones with an average insert size of 57 kb and represents about 4 maize haploid genome equivalents. Characterization of the library showed less than 0.5% of clones to not contain large inserts. Significant contamination of chloroplast and mitochondria DNA was not detected. BAC clones (152 arrays) were stored in 96 microtiter plates, with each well containing 12 clones. This is the first maize BAC library constructed in China. It is well suited for map-based cloning of maize genes and genome physical mapping.

KW - 77Ht2

KW - Bacterial artificial chromosome

KW - Maize

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DO - 10.1007/BF02774242

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SN - 0735-9640

VL - 21

SP - 159

EP - 169

JO - Plant Molecular Biology Reporter

JF - Plant Molecular Biology Reporter

IS - 2

ER -

Construction and characterization of a bacterial artificial chromosome library of maize inbred line 77Ht2

Abstract

All Science Journal Classification (ASJC) codes

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