Construction and characterization of a bacterial artificial chromosome library of maize inbred line 77Ht2

Jiliang Yang, Qinghua Wang, Daiyong Deng, Manli Weng, Xiaoyan Yin, Demin Jin, Juren Zhang, Bin Wang

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Maize inbred line 77Ht2 contains agriculturally important genes and has been widely used in corn breeding in China. A bacterial artificial chromosome (BAC) library of 77Ht2 has been constructed in order to identify useful genes and to facilitate the study of the maize genome. The library contains 175104 clones with an average insert size of 57 kb and represents about 4 maize haploid genome equivalents. Characterization of the library showed less than 0.5% of clones to not contain large inserts. Significant contamination of chloroplast and mitochondria DNA was not detected. BAC clones (152 arrays) were stored in 96 microtiter plates, with each well containing 12 clones. This is the first maize BAC library constructed in China. It is well suited for map-based cloning of maize genes and genome physical mapping.

Original languageEnglish (US)
Pages (from-to)159-169
Number of pages11
JournalPlant Molecular Biology Reporter
Volume21
Issue number2
DOIs
StatePublished - Jun 2003
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Plant Science

Keywords

  • 77Ht2
  • Bacterial artificial chromosome
  • Maize

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