Coupling of ultrafast LC with mass spectrometry by DESI

Yi Cai, Yong Liu, Roy Helmy, Hao Chen

Research output: Contribution to journalArticlepeer-review

12 Scopus citations


Recently we reported a desorption electrospray ionization (DESI) interface to combine liquid chromatography (LC) with mass spectrometry (MS) using a new LC eluent splitting strategy through a tiny orifice on LC capillary tube [J. Am. Soc. Mass Spectrom. 25, 286 (2014)]. The interface introduces negligible dead volume and back pressure, thereby allowing "near real-time" MS detection, fast LC elution, and online MS-directed purification. This study further evaluates the LC/DESI-MS performance with focus of using ultra-fast LC. Using a monolithic C18 column, metabolites in urine can be separated within 1.6 min and can be online collected for subsequent structure elucidation (e.g., by NMR, UV, IR) in a recovery yield up to 99%. Using a spray solvent with alkaline pH, negative ions could be directly generated for acidic analytes (e.g., ibuprofen) in acidic LC eluent by DESI, offering a novel protocol to realize "wrong-way around" ionization for LC/MS analysis. In addition, DESI-MS is found to be compatible with ultra-performance liquid chromatography (UPLC) for the first time.

Original languageEnglish (US)
Pages (from-to)1820-1823
Number of pages4
JournalJournal of the American Society for Mass Spectrometry
Issue number10
StatePublished - Oct 2014
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Spectroscopy


  • DESI
  • LC/MS
  • Monolithic column
  • UPLC


Dive into the research topics of 'Coupling of ultrafast LC with mass spectrometry by DESI'. Together they form a unique fingerprint.

Cite this