TY - JOUR
T1 - Decolorization of anthraquinone, triphenylmethane and azo dyes by a new isolated Bacillus cereus strain DC11
AU - Deng, Daiyong
AU - Guo, Jun
AU - Zeng, Guoqu
AU - Sun, Guoping
N1 - Funding Information:
This work was supported by the fund of Chinese National Programs for High Technology Research and Development (No. 2006AA06Z322).
PY - 2008/10
Y1 - 2008/10
N2 - In this study, we isolated and characterized a new strain of Bacillus cereus, DC11, capable of decolorizing a broad-spectrum of dyes. Parameters including pH, temperature, oxygen concentration and carbon source were used to comparatively study the decolorizing effects on anthraquinone, triphenylmethane, and azo dyes, respectively. High decolorization efficiency (95-98%) was achieved within 6 h of incubation for 100 μM Acid Blue 25 (anthraquinone dye), 4 h for 55 μM Malachite Green (triphenylmethane dye), and 2 h for 750 μM Basic Blue X-GRRL (azo dye) at 20-45 °C and neutral pH under anaerobic conditions. Decolorizing activities, which showed NADH/NADPH-dependent traits were limited to the soluble cytosolic fractions. Among the decolorized products, there was an intermediate metabolite after the degradation of Acid Blue 25 dye. Malachite Green was degraded into 4,4′-bis(dimethylamino)benzophenone and benzophenone, and the decolorization of Basic Blue X-GRRL was probably due to the reduction of the azo bonds. Our new isolated strain DC11 is the first reported Bacillus cereus strain which can efficiently decolorize all three main group dyes, and the converging parameters for the decolorization of three group dyes may be useful for bioremediation applications.
AB - In this study, we isolated and characterized a new strain of Bacillus cereus, DC11, capable of decolorizing a broad-spectrum of dyes. Parameters including pH, temperature, oxygen concentration and carbon source were used to comparatively study the decolorizing effects on anthraquinone, triphenylmethane, and azo dyes, respectively. High decolorization efficiency (95-98%) was achieved within 6 h of incubation for 100 μM Acid Blue 25 (anthraquinone dye), 4 h for 55 μM Malachite Green (triphenylmethane dye), and 2 h for 750 μM Basic Blue X-GRRL (azo dye) at 20-45 °C and neutral pH under anaerobic conditions. Decolorizing activities, which showed NADH/NADPH-dependent traits were limited to the soluble cytosolic fractions. Among the decolorized products, there was an intermediate metabolite after the degradation of Acid Blue 25 dye. Malachite Green was degraded into 4,4′-bis(dimethylamino)benzophenone and benzophenone, and the decolorization of Basic Blue X-GRRL was probably due to the reduction of the azo bonds. Our new isolated strain DC11 is the first reported Bacillus cereus strain which can efficiently decolorize all three main group dyes, and the converging parameters for the decolorization of three group dyes may be useful for bioremediation applications.
KW - Bacillus cereus strain DC11
KW - Broad-spectrum
KW - Comparative analysis
KW - Decolorization and degradation
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U2 - 10.1016/j.ibiod.2008.01.017
DO - 10.1016/j.ibiod.2008.01.017
M3 - Article
AN - SCOPUS:50849109168
SN - 0964-8305
VL - 62
SP - 263
EP - 269
JO - International Biodeterioration and Biodegradation
JF - International Biodeterioration and Biodegradation
IS - 3
ER -