@article{256db9f92f7c4b2694a44291e9ac7bc2,
title = "Detection of cancer antigens (CA-125) using gold nano particles on interdigitated electrode-based microfluidic biosensor",
abstract = "Integrating microfluidics with biosensors is of great research interest with the increasing trend of lab-on-the chip and point-of-care devices. Though there have been numerous studies performed relating microfluidics to the biosensing mechanisms, the study of the sensitivity variation due to microfluidic flow is very much limited. In this paper, the sensitivity of interdigitated electrodes was evaluated at the static drop condition and the microfluidic flow condition. In addition, this study demonstrates the use of gold nanoparticles to enhance the sensor signal response and provides experimental results of the capacitance difference during cancer antigen-125 (CA-125) antigen–antibody conjugation at multiple concentrations of CA-125 antigens. The experimental results also provide evidence of disease-specific detection of CA-125 antigen at multiple concentrations with the increase in capacitive signal response proportional to the concentration of the CA-125 antigens. The capacitive signal response of antigen–antibody conjugation on interdigitate electrodes has been enhanced by approximately 2.8 times (from 260.80 to 736.33 pF at 20 kHz frequency) in static drop condition and approximately 2.5 times (from 205.85 to 518.48 pF at 20 kHz frequency) in microfluidic flow condition with gold nanoparticle-coating. The capacitive signal response is observed to decrease at microfluidic flow condition at both plain interdigitated electrodes (from 260.80 to 205.85 pF at 20 kHz frequency) and gold nano particle coated interdigitated electrodes (from 736.33 to 518.48 pF at 20 kHz frequency), due to the strong shear effect compared to static drop condition. However, the microfluidic channel in the biosensor has the potential to increase the signal to noise ratio due to plasma separation from the whole blood and lead to the increase concentration of the biomarkers in the blood volume for sensing.",
keywords = "Biosensor, Capacitance measurements, Gold nanoparticles, Interdigitated gold electrodes, Microfluidic channel",
author = "Nunna, {Bharath Babu} and Debdyuti Mandal and Lee, {Joo Un} and Harsimranjit Singh and Shiqiang Zhuang and Durgamadhab Misra and Bhuyian, {Md Nasir Uddin} and Lee, {Eon Soo}",
note = "Funding Information: The authors acknowledge the research support from New Jersey Institute of Technology (NJIT). This research is carried out in part at the Center for Functional Nanomaterials, Brookhaven National Laboratory, which is supported by the U.S. Department of Energy, Office of Basic Energy Sciences, under Contract No. DE-SC0012704. Funding Information: This research is supported by the National Science Foundation Fund (Grant ID: NSF IIP-1643861). Funding Information: BBN and DeM have contributed to sample preparation, data curation, data analysis, and original draft writing. BBN and ESL have contributed for review and editing the manuscript. JUL has contributed to procuring experimental resources, data acquisition and analysis. HS and SZ have contributed to procuring the resources and samples, and reviewing and editing the manuscript according to journal specifications. DuM, MNUB and BBN have contributed to the software support for acquiring and managing the experimental data. ESL has designed and supervised the biosensor project along with advising and reviewing the manuscript. All authors read and approved the final manuscript. The authors acknowledge the research support from New Jersey Institute of Technology (NJIT). This research is carried out in part at the Center for Functional Nanomaterials, Brookhaven National Laboratory, which is supported by the U.S. Department of Energy, Office of Basic Energy Sciences, under Contract No. DE-SC0012704. The authors declare that they have no competing interests. The datasets used and/or analysed during the current study are available from the corresponding author on reasonable request. This research is supported by the National Science Foundation Fund (Grant ID: NSF IIP-1643861). Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Publisher Copyright: {\textcopyright} 2019, The Author(s).",
year = "2019",
month = dec,
day = "1",
doi = "10.1186/s40580-019-0173-6",
language = "English (US)",
volume = "6",
journal = "Nano Convergence",
issn = "2196-5404",
publisher = "Springer Open",
number = "1",
}