Developing a tissue-engineered neural-electrical relay using encapsulated neuronal constructs on conducting polymer fibers

D. Kacy Cullen, Ankur R. Patel, John F. Doorish, Douglas H. Smith, Bryan J. Pfister

Research output: Contribution to journalArticlepeer-review

56 Scopus citations

Abstract

Neural-electrical interface platforms are being developed to extracellularly monitor neuronal population activity. Polyaniline-based electrically conducting polymer fibers are attractive substrates for sustained functional interfaces with neurons due to their flexibility, tailored geometry and controlled electro-conductive properties. In this study, we addressed the neurobiological considerations of utilizing small diameter (<400 νm) fibers consisting of a blend of electrically conductive polyaniline and polypropylene (PA-PP) as the backbone of encapsulated tissue-engineered neural-electrical relays. We devised new approaches to promote survival, adhesion and neurite outgrowth of primary dorsal root ganglion neurons on PA-PP fibers. We attained a greater than ten-fold increase in the density of viable neurons on fiber surfaces to approximately 700 neurons mm-2 by manipulating surrounding surface charges to bias settling neuronal suspensions toward fibers coated with cell-adhesive ligands. This stark increase in neuronal density resulted in robust neuritic extension and network formation directly along the fibers. Additionally, we encapsulated these neuronal networks on PA-PP fibers using agarose to form a protective barrier while potentially facilitating network stability. Following encapsulation, the neuronal networks maintained integrity, high viability (>85%) and intimate adhesion to PA-PP fibers. These efforts accomplished key prerequisites for the establishment of functional electrical interfaces with neuronal populations using small diameter PA-PP fibers - specifically, improved neurocompatibility, high-density neuronal adhesion and neuritic network development directly on fiber surfaces.

Original languageEnglish (US)
Pages (from-to)374-384
Number of pages11
JournalJournal of neural engineering
Volume5
Issue number4
DOIs
StatePublished - 2008
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering
  • Cellular and Molecular Neuroscience

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