Development and characterization of a bioglass/chitosan composite as an injectable bone substitute

Parastoo Khoshakhlagh, Sayed Mahmood Rabiee, Gita Kiaee, Pedram Heidari, Amir K. Miri, Roshanak Moradi, Fathollah Moztarzadeh, Roya Ravarian

Research output: Contribution to journalArticlepeer-review

51 Scopus citations


SiO2-CaO-P2O5 based bioglass (BG) systems constitute a group of materials that have wide applications in bone implants. Chitosan (Cn) is a biocompatible and osteoconductive natural polymer that can promote wound healing. In this study, bioactivity of chitosan/bioglass (CnB) composites as minimally invasive bone regenerative materials was assessed both in vitro and in vivo. Injectability tests and scanning electron microscopy (SEM) results demonstrated the formation of uniform injectable paste-like composites using BG particles and Cn. Fourier transform infrared spectroscopy (FTIR) and SEM images confirmed hydroxyapatite deposition in vitro after incubation in simulated body fluid (SBF). Higher BG content in the composite correlated with increased human osteoblast proliferation. An in vivo study in a rat spinal fusion model confirmed that increasing the amount of BG improved osteoconductivity. Manual palpation, radiographic images and pathological assessments proved that the composites promote bone formation. Based on these data, the synthesized composites have a potential application in orthopedic and reconstructive surgeries as a minimally invasive bone substitute.

Original languageEnglish (US)
Pages (from-to)1261-1271
Number of pages11
JournalCarbohydrate Polymers
StatePublished - Feb 10 2017
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Organic Chemistry
  • Polymers and Plastics
  • Materials Chemistry


  • Bone tissue engineering
  • Chitosan
  • In vivo bone regeneration
  • Polymer/bioglass composites


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