Development of multinuclear polymeric nanoparticles as robust protein nanocarriers

Jun Wu, Nazila Kamaly, Jinjun Shi, Lili Zhao, Zeyu Xiao, Geoffrey Hollett, Rohit John, Shaunak Ray, Xiaoyang Xu, Xueqing Zhang, Philip W. Kantoff, Omid C. Farokhzad

Research output: Contribution to journalArticlepeer-review

127 Scopus citations


One limitation of current biodegradable polymeric nanoparticles is their inability to effectively encapsulate and sustainably release proteins while maintaining protein bioactivity. Here we report the engineering of PLGA-polycation nanoparticles with a core-shell structure that act as a robust vector for the encapsulation and delivery of proteins and peptides. The optimized nanoparticles can load high amounts of proteins (>20% of nanoparticles by weight) in aqueous solution without organic solvents through electrostatic interactions by simple mixing, thereby forming nanospheres in seconds with diameters <200 nm. The relationship between nanosphere size, surface charge, PLGA-polycation composition, and protein loading is also investigated. The stable nanosphere complexes contain multiple PLGA-polycation nanoparticles, surrounded by large amounts of protein. This study highlights a novel strategy for the delivery of proteins and other relevant molecules.

Original languageEnglish (US)
Pages (from-to)8975-8979
Number of pages5
JournalAngewandte Chemie - International Edition
Issue number34
StatePublished - Aug 18 2014
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Catalysis
  • General Chemistry


  • nanoparticles
  • polycations
  • polymers
  • protein delivery
  • structure-function relationships


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