Differential effects of ketamine/xylazine anesthesia on the cerebral and cerebellar cortical activities in the rat

Gokhan Ordek, Jonathan D. Groth, Mesut Sahin

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Cerebellum is a highly organized structure with a crystalline morphology that has always intrigued neuroscientists. Much of the cerebellar research has been conducted in anesthetized animals, particularly using ketamine/xylazine combination in rats. It is not clear how and to what extent the cerebellar cortical circuitry is affected by this anesthesia. In this study, we recorded spontaneous and evoked potentials from the cerebellar surface with chronically implanted, flexible-substrate, multielectrode arrays in rats and compared them to the signals simultaneously recorded from the motor cortex with similar electrodes. The power spectra and the intercontact coherence plots of the spontaneous activity in the awake-quiet animals extended up to 800 Hz in the cerebellum and only up to 200 Hz in the motor cortex. Ketamine/xylazine anesthesia suppressed most of the activity in the cerebellar cortex, which was in clear contrast to the motor cortex. In the awake cerebellum, large coherence values were observed between contact pairs as far apart as ~2 mm. Otherwise, there was not a discernable relation between the coherence and the intercontact distance. These results suggest that the surface electrodes can provide much more detailed information about the state of neural circuits when they are used on the cerebellar cortex compared with the cerebral areas. This may be due to the proximity of the molecular layer cells to the pial surface in the cerebellum.

Original languageEnglish (US)
Pages (from-to)1435-1443
Number of pages9
JournalJournal of neurophysiology
Volume109
Issue number5
DOIs
StatePublished - 2013

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Physiology

Keywords

  • Cerebellum and anesthesia
  • Evoked potentials
  • Micro-ECoG
  • Multielectrode arrays

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