TY - JOUR
T1 - Effect of crosslinking, hydroxyapatite addition, and fiber alignment to stimulate human mesenchymal stem cells osteoinduction in polycaprolactone-based electrospun scaffolds
AU - Menezes, Felipe Castro
AU - Siqueira, Nataly Machado
AU - Fung, Stephanie
AU - Scheibel, Jóice Maria
AU - Moura, Dinara Jaqueline
AU - Guvendiren, Murat
AU - Kohn, Joachim
AU - Soares, Rosane Michele Duarte
N1 - Publisher Copyright:
© 2022 John Wiley & Sons Ltd.
PY - 2022/9
Y1 - 2022/9
N2 - Electrospinning is a versatile technique for producing composite scaffolds with nanostructure properties similar to the natural extracellular matrix. Biomaterials possessing mechanical, structural, and biological properties required for bone tissue engineering are a big challenge. However, the effect of fiber alignment, their mechanical properties, and chemical modifications on fibers are usually investigated individually. In this study, PCL/GE/HA scaffolds were electrospun in a static and drum rotatory collector to investigate the effects of alignment on the physicochemical properties of composite scaffolds. Furthermore, to achieve a stable composite with natural polymer gelatin (GE), a water-soluble, zero-length crosslinker (N-[3-dimethylaminopropyl]-N′-ethylcarbodiimide hydrochloride, EDC) was used to crosslink GE. Our results have outlined that the incorporation of GE, as well as crosslinking process, produced a hydrophilic biomaterial, improving wettability compared to pure polycaprolactone (PCL). In addition, the alignment reinforced the material, increasing mechanical strength. Biological tests showed that GE addition and the alignment allowed a better osteoinduction than pure random PCL.
AB - Electrospinning is a versatile technique for producing composite scaffolds with nanostructure properties similar to the natural extracellular matrix. Biomaterials possessing mechanical, structural, and biological properties required for bone tissue engineering are a big challenge. However, the effect of fiber alignment, their mechanical properties, and chemical modifications on fibers are usually investigated individually. In this study, PCL/GE/HA scaffolds were electrospun in a static and drum rotatory collector to investigate the effects of alignment on the physicochemical properties of composite scaffolds. Furthermore, to achieve a stable composite with natural polymer gelatin (GE), a water-soluble, zero-length crosslinker (N-[3-dimethylaminopropyl]-N′-ethylcarbodiimide hydrochloride, EDC) was used to crosslink GE. Our results have outlined that the incorporation of GE, as well as crosslinking process, produced a hydrophilic biomaterial, improving wettability compared to pure polycaprolactone (PCL). In addition, the alignment reinforced the material, increasing mechanical strength. Biological tests showed that GE addition and the alignment allowed a better osteoinduction than pure random PCL.
KW - bone tissue engineering
KW - electrospinning
KW - gelatin
KW - hydroxyapatite
KW - polycaprolactone
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U2 - 10.1002/pat.5723
DO - 10.1002/pat.5723
M3 - Article
AN - SCOPUS:85129633582
SN - 1042-7147
VL - 33
SP - 2682
EP - 2695
JO - Polymers for Advanced Technologies
JF - Polymers for Advanced Technologies
IS - 9
ER -