Electrochemical DNA biosensor for the detection of specific gene related to Microcystis species

Fei Yan, Arzum Erdem, Burcu Meric, Kagan Kerman, Mehmet Ozsoz, Omowunmi A. Sadik

Research output: Contribution to journalArticlepeer-review

86 Scopus citations

Abstract

A new electrochemical biosensor is described for voltammetric detection of gene sequence related to bloom-forming genera of cyanobacteria, Microcystis spp. The sensor involves the immobilization of a 17-mer DNA probe, which is complementary to a specific gene sequence related to Microcystis spp. on a gold electrode through specific adsorption. The DNA probe was used to determine the amount of target gene in solution using methylene blue (MB) and ruthenium bipyridine as the electrochemical indicators. The anodic peak currents (ipa) of Ru(bpy)32+ were linearly related to the concentration of the target oligonucleotide sequence in the range 1.8 × 10-10-9.0 × 10-8 M. The detection limit of this approach was 9.0 × 10-11 M. In addition, these indicators were capable of selectively discriminating against mismatches; a very desirable condition for the detection of disease-related point-mutation in guanine bases of the cyanobacteria.

Original languageEnglish (US)
Pages (from-to)224-228
Number of pages5
JournalElectrochemistry Communications
Volume3
Issue number5
DOIs
StatePublished - May 2001
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Electrochemistry

Keywords

  • DNA hybridization
  • Methylene blue
  • Microcystis spp.
  • Ruthenium bipyridine

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