Engineering three-dimensional nervous tissue constructs based on fiber-gel substrates

M. L. Siriwardane; Bryan Pfister

doi:10.1109/NEBC.2010.5458119

Engineering three-dimensional nervous tissue constructs based on fiber-gel substrates

M. L. Siriwardane, Bryan Pfister

Bio-Medical Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

1 Scopus citations

Abstract

Three-dimensional (3D) nervous tissue constructs were developed using a compliant gel matrix and a fiber component for promoting contact-guided axon outgrowth. Rat embryonic (E15) dorsal root ganglion explants (DRGs) were cultured on biodegradable poly-L-lactic acid (PLLA) and wet-spun type I collagen fibers embedded in soft collagen gels. Gel constructs containing fibers induced greater axon outgrowth distances with significantly more directionality along the aligned fibers. Interestingly, wet-spun type I collagen from rat tail tendon displayed a highly aligned outgrowth pattern from DRG axons suggesting potential use for peripheral nerve repair.

Original language	English (US)
Title of host publication	Proceedings of the 2010 IEEE 36th Annual Northeast Bioengineering Conference, NEBEC 2010
DOIs	https://doi.org/10.1109/NEBC.2010.5458119
State	Published - Jun 8 2010
Event	36th Annual Northeast Bioengineering Conference, NEBEC 2010 - New York, NY, United States Duration: Mar 26 2010 → Mar 28 2010

Publication series

Name	Proceedings of the 2010 IEEE 36th Annual Northeast Bioengineering Conference, NEBEC 2010

Other

Other	36th Annual Northeast Bioengineering Conference, NEBEC 2010
Country/Territory	United States
City	New York, NY
Period	3/26/10 → 3/28/10

All Science Journal Classification (ASJC) codes

Bioengineering

Access to Document

10.1109/NEBC.2010.5458119

Cite this

@inproceedings{517ed21210be468ba78966a6deb31649,

title = "Engineering three-dimensional nervous tissue constructs based on fiber-gel substrates",

abstract = "Three-dimensional (3D) nervous tissue constructs were developed using a compliant gel matrix and a fiber component for promoting contact-guided axon outgrowth. Rat embryonic (E15) dorsal root ganglion explants (DRGs) were cultured on biodegradable poly-L-lactic acid (PLLA) and wet-spun type I collagen fibers embedded in soft collagen gels. Gel constructs containing fibers induced greater axon outgrowth distances with significantly more directionality along the aligned fibers. Interestingly, wet-spun type I collagen from rat tail tendon displayed a highly aligned outgrowth pattern from DRG axons suggesting potential use for peripheral nerve repair.",

author = "Siriwardane, {M. L.} and Bryan Pfister",

year = "2010",

month = jun,

day = "8",

doi = "10.1109/NEBC.2010.5458119",

language = "English (US)",

isbn = "9781424468799",

series = "Proceedings of the 2010 IEEE 36th Annual Northeast Bioengineering Conference, NEBEC 2010",

booktitle = "Proceedings of the 2010 IEEE 36th Annual Northeast Bioengineering Conference, NEBEC 2010",

note = "36th Annual Northeast Bioengineering Conference, NEBEC 2010 ; Conference date: 26-03-2010 Through 28-03-2010",

}

Siriwardane, ML & Pfister, B 2010, Engineering three-dimensional nervous tissue constructs based on fiber-gel substrates. in Proceedings of the 2010 IEEE 36th Annual Northeast Bioengineering Conference, NEBEC 2010., 5458119, Proceedings of the 2010 IEEE 36th Annual Northeast Bioengineering Conference, NEBEC 2010, 36th Annual Northeast Bioengineering Conference, NEBEC 2010, New York, NY, United States, 3/26/10. https://doi.org/10.1109/NEBC.2010.5458119

Engineering three-dimensional nervous tissue constructs based on fiber-gel substrates. / Siriwardane, M. L.; Pfister, Bryan.

Proceedings of the 2010 IEEE 36th Annual Northeast Bioengineering Conference, NEBEC 2010. 2010. 5458119 (Proceedings of the 2010 IEEE 36th Annual Northeast Bioengineering Conference, NEBEC 2010).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - Engineering three-dimensional nervous tissue constructs based on fiber-gel substrates

AU - Siriwardane, M. L.

AU - Pfister, Bryan

PY - 2010/6/8

Y1 - 2010/6/8

N2 - Three-dimensional (3D) nervous tissue constructs were developed using a compliant gel matrix and a fiber component for promoting contact-guided axon outgrowth. Rat embryonic (E15) dorsal root ganglion explants (DRGs) were cultured on biodegradable poly-L-lactic acid (PLLA) and wet-spun type I collagen fibers embedded in soft collagen gels. Gel constructs containing fibers induced greater axon outgrowth distances with significantly more directionality along the aligned fibers. Interestingly, wet-spun type I collagen from rat tail tendon displayed a highly aligned outgrowth pattern from DRG axons suggesting potential use for peripheral nerve repair.

AB - Three-dimensional (3D) nervous tissue constructs were developed using a compliant gel matrix and a fiber component for promoting contact-guided axon outgrowth. Rat embryonic (E15) dorsal root ganglion explants (DRGs) were cultured on biodegradable poly-L-lactic acid (PLLA) and wet-spun type I collagen fibers embedded in soft collagen gels. Gel constructs containing fibers induced greater axon outgrowth distances with significantly more directionality along the aligned fibers. Interestingly, wet-spun type I collagen from rat tail tendon displayed a highly aligned outgrowth pattern from DRG axons suggesting potential use for peripheral nerve repair.

UR - http://www.scopus.com/inward/record.url?scp=77953063919&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77953063919&partnerID=8YFLogxK

U2 - 10.1109/NEBC.2010.5458119

DO - 10.1109/NEBC.2010.5458119

M3 - Conference contribution

AN - SCOPUS:77953063919

SN - 9781424468799

T3 - Proceedings of the 2010 IEEE 36th Annual Northeast Bioengineering Conference, NEBEC 2010

BT - Proceedings of the 2010 IEEE 36th Annual Northeast Bioengineering Conference, NEBEC 2010

T2 - 36th Annual Northeast Bioengineering Conference, NEBEC 2010

Y2 - 26 March 2010 through 28 March 2010

ER -

Engineering three-dimensional nervous tissue constructs based on fiber-gel substrates

Abstract

Publication series

Other

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this