TY - GEN
T1 - Engineering three-dimensional nervous tissue constructs based on fiber-gel substrates
AU - Siriwardane, M. L.
AU - Pfister, Bryan
PY - 2010/6/8
Y1 - 2010/6/8
N2 - Three-dimensional (3D) nervous tissue constructs were developed using a compliant gel matrix and a fiber component for promoting contact-guided axon outgrowth. Rat embryonic (E15) dorsal root ganglion explants (DRGs) were cultured on biodegradable poly-L-lactic acid (PLLA) and wet-spun type I collagen fibers embedded in soft collagen gels. Gel constructs containing fibers induced greater axon outgrowth distances with significantly more directionality along the aligned fibers. Interestingly, wet-spun type I collagen from rat tail tendon displayed a highly aligned outgrowth pattern from DRG axons suggesting potential use for peripheral nerve repair.
AB - Three-dimensional (3D) nervous tissue constructs were developed using a compliant gel matrix and a fiber component for promoting contact-guided axon outgrowth. Rat embryonic (E15) dorsal root ganglion explants (DRGs) were cultured on biodegradable poly-L-lactic acid (PLLA) and wet-spun type I collagen fibers embedded in soft collagen gels. Gel constructs containing fibers induced greater axon outgrowth distances with significantly more directionality along the aligned fibers. Interestingly, wet-spun type I collagen from rat tail tendon displayed a highly aligned outgrowth pattern from DRG axons suggesting potential use for peripheral nerve repair.
UR - http://www.scopus.com/inward/record.url?scp=77953063919&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77953063919&partnerID=8YFLogxK
U2 - 10.1109/NEBC.2010.5458119
DO - 10.1109/NEBC.2010.5458119
M3 - Conference contribution
AN - SCOPUS:77953063919
SN - 9781424468799
T3 - Proceedings of the 2010 IEEE 36th Annual Northeast Bioengineering Conference, NEBEC 2010
BT - Proceedings of the 2010 IEEE 36th Annual Northeast Bioengineering Conference, NEBEC 2010
T2 - 36th Annual Northeast Bioengineering Conference, NEBEC 2010
Y2 - 26 March 2010 through 28 March 2010
ER -