Enhanced fluorescence through the incorporation of nanocones/gaps into a plasmonic gratings sensor platform

Aaron Wood; Sheila Grant; Sagnik Basuray; Avinash Pathak; Sangho Bok; Cherian Mathai; Keshab Gangopadhyay; Shubhra Gangopadhyay

doi:10.1109/ICSENS.2014.6985294

Enhanced fluorescence through the incorporation of nanocones/gaps into a plasmonic gratings sensor platform

Aaron Wood, Sheila Grant, Sagnik Basuray, Avinash Pathak, Sangho Bok, Cherian Mathai, Keshab Gangopadhyay, Shubhra Gangopadhyay

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

3 Scopus citations

Abstract

In this article, a novel plasmonic grating sensor platform was developed and tested for feasibility in sensor applications using a "lights-on" fluorescence based DNA sensor. The sensor platform combined the fluorescence enhancement of a grating-based plasmonic platform with the electric field intensifying effects of nano-scale cones and cavities. The gratings were made through a microcontact printing process that replicated HD-DVD discs in polymethylsilsesquioxane (PMSSQ) and coated in a thin gold film. Nanocavities were incorporated into the sensor platform during the printing process and nanocones were incorporated during the 100 nm gold deposition process. Fluorescently-tagged single-strand (SS) DNA molecules were immobilized onto the surface and were designed such that the molecules would fluoresce when bound to a complementary sequence. Sensor substrates were imaged after exposure to a mismatched and matched oligomer to quantify the fluorescence enhancement of the sensor. Much higher fluorescence intensity was observed on all of the plasmonic structures as compared to flat gold.

Original language	English (US)
Title of host publication	IEEE SENSORS 2014, Proceedings
Editors	Francisco J. Arregui
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	1479-1482
Number of pages	4
Edition	December
ISBN (Electronic)	9781479901616
DOIs	https://doi.org/10.1109/ICSENS.2014.6985294
State	Published - Dec 12 2014
Externally published	Yes
Event	13th IEEE SENSORS Conference, SENSORS 2014 - Valencia, Spain Duration: Nov 2 2014 → Nov 5 2014

Publication series

Name	Proceedings of IEEE Sensors
Number	December
Volume	2014-December
ISSN (Print)	1930-0395
ISSN (Electronic)	2168-9229

Conference

Conference	13th IEEE SENSORS Conference, SENSORS 2014
Country/Territory	Spain
City	Valencia
Period	11/2/14 → 11/5/14

All Science Journal Classification (ASJC) codes

Electrical and Electronic Engineering

Keywords

Aptamer
Fluorescence
Grating
Microcontact
Nanocavity
Nanocone
Plasmonic

Access to Document

10.1109/ICSENS.2014.6985294

Cite this

Wood, A., Grant, S., Basuray, S., Pathak, A., Bok, S., Mathai, C., Gangopadhyay, K., & Gangopadhyay, S. (2014). Enhanced fluorescence through the incorporation of nanocones/gaps into a plasmonic gratings sensor platform. In F. J. Arregui (Ed.), IEEE SENSORS 2014, Proceedings (December ed., pp. 1479-1482). [6985294] (Proceedings of IEEE Sensors; Vol. 2014-December, No. December). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/ICSENS.2014.6985294

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title = "Enhanced fluorescence through the incorporation of nanocones/gaps into a plasmonic gratings sensor platform",

abstract = "In this article, a novel plasmonic grating sensor platform was developed and tested for feasibility in sensor applications using a {"}lights-on{"} fluorescence based DNA sensor. The sensor platform combined the fluorescence enhancement of a grating-based plasmonic platform with the electric field intensifying effects of nano-scale cones and cavities. The gratings were made through a microcontact printing process that replicated HD-DVD discs in polymethylsilsesquioxane (PMSSQ) and coated in a thin gold film. Nanocavities were incorporated into the sensor platform during the printing process and nanocones were incorporated during the 100 nm gold deposition process. Fluorescently-tagged single-strand (SS) DNA molecules were immobilized onto the surface and were designed such that the molecules would fluoresce when bound to a complementary sequence. Sensor substrates were imaged after exposure to a mismatched and matched oligomer to quantify the fluorescence enhancement of the sensor. Much higher fluorescence intensity was observed on all of the plasmonic structures as compared to flat gold.",

keywords = "Aptamer, Fluorescence, Grating, Microcontact, Nanocavity, Nanocone, Plasmonic",

author = "Aaron Wood and Sheila Grant and Sagnik Basuray and Avinash Pathak and Sangho Bok and Cherian Mathai and Keshab Gangopadhyay and Shubhra Gangopadhyay",

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year = "2014",

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Wood, A, Grant, S, Basuray, S, Pathak, A, Bok, S, Mathai, C, Gangopadhyay, K & Gangopadhyay, S 2014, Enhanced fluorescence through the incorporation of nanocones/gaps into a plasmonic gratings sensor platform. in FJ Arregui (ed.), IEEE SENSORS 2014, Proceedings. December edn, 6985294, Proceedings of IEEE Sensors, no. December, vol. 2014-December, Institute of Electrical and Electronics Engineers Inc., pp. 1479-1482, 13th IEEE SENSORS Conference, SENSORS 2014, Valencia, Spain, 11/2/14. https://doi.org/10.1109/ICSENS.2014.6985294

Enhanced fluorescence through the incorporation of nanocones/gaps into a plasmonic gratings sensor platform. / Wood, Aaron; Grant, Sheila; Basuray, Sagnik et al.

IEEE SENSORS 2014, Proceedings. ed. / Francisco J. Arregui. December. ed. Institute of Electrical and Electronics Engineers Inc., 2014. p. 1479-1482 6985294 (Proceedings of IEEE Sensors; Vol. 2014-December, No. December).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

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AU - Grant, Sheila

AU - Basuray, Sagnik

AU - Pathak, Avinash

AU - Bok, Sangho

AU - Mathai, Cherian

AU - Gangopadhyay, Keshab

AU - Gangopadhyay, Shubhra

PY - 2014/12/12

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N2 - In this article, a novel plasmonic grating sensor platform was developed and tested for feasibility in sensor applications using a "lights-on" fluorescence based DNA sensor. The sensor platform combined the fluorescence enhancement of a grating-based plasmonic platform with the electric field intensifying effects of nano-scale cones and cavities. The gratings were made through a microcontact printing process that replicated HD-DVD discs in polymethylsilsesquioxane (PMSSQ) and coated in a thin gold film. Nanocavities were incorporated into the sensor platform during the printing process and nanocones were incorporated during the 100 nm gold deposition process. Fluorescently-tagged single-strand (SS) DNA molecules were immobilized onto the surface and were designed such that the molecules would fluoresce when bound to a complementary sequence. Sensor substrates were imaged after exposure to a mismatched and matched oligomer to quantify the fluorescence enhancement of the sensor. Much higher fluorescence intensity was observed on all of the plasmonic structures as compared to flat gold.

AB - In this article, a novel plasmonic grating sensor platform was developed and tested for feasibility in sensor applications using a "lights-on" fluorescence based DNA sensor. The sensor platform combined the fluorescence enhancement of a grating-based plasmonic platform with the electric field intensifying effects of nano-scale cones and cavities. The gratings were made through a microcontact printing process that replicated HD-DVD discs in polymethylsilsesquioxane (PMSSQ) and coated in a thin gold film. Nanocavities were incorporated into the sensor platform during the printing process and nanocones were incorporated during the 100 nm gold deposition process. Fluorescently-tagged single-strand (SS) DNA molecules were immobilized onto the surface and were designed such that the molecules would fluoresce when bound to a complementary sequence. Sensor substrates were imaged after exposure to a mismatched and matched oligomer to quantify the fluorescence enhancement of the sensor. Much higher fluorescence intensity was observed on all of the plasmonic structures as compared to flat gold.

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KW - Nanocavity

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M3 - Conference contribution

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T3 - Proceedings of IEEE Sensors

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EP - 1482

BT - IEEE SENSORS 2014, Proceedings

A2 - Arregui, Francisco J.

PB - Institute of Electrical and Electronics Engineers Inc.

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ER -

Wood A, Grant S, Basuray S, Pathak A, Bok S, Mathai C et al. Enhanced fluorescence through the incorporation of nanocones/gaps into a plasmonic gratings sensor platform. In Arregui FJ, editor, IEEE SENSORS 2014, Proceedings. December ed. Institute of Electrical and Electronics Engineers Inc. 2014. p. 1479-1482. 6985294. (Proceedings of IEEE Sensors; December). doi: 10.1109/ICSENS.2014.6985294

Enhanced fluorescence through the incorporation of nanocones/gaps into a plasmonic gratings sensor platform

Abstract

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All Science Journal Classification (ASJC) codes

Keywords

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