TY - JOUR
T1 - Enhanced fluorescence through the incorporation of nanocones/gaps into a plasmonic gratings sensor platform
AU - Wood, Aaron
AU - Grant, Sheila
AU - Basuray, Sagnik
AU - Pathak, Avinash
AU - Bok, Sangho
AU - Mathai, Cherian
AU - Gangopadhyay, Keshab
AU - Gangopadhyay, Shubhra
PY - 2014/1/1
Y1 - 2014/1/1
N2 - In this article, a novel plasmonic grating sensor platform was developed and tested for feasibility in sensor applications using a "lights-on" fluorescence based DNA sensor. The sensor platform combined the fluorescence enhancement of a grating-based plasmonic platform with the electric field intensifying effects of nano-scale cones and cavities. The gratings were made through a microcontact printing process that replicated HD-DVD discs in polymethylsilsesquioxane (PMSSQ) and coated in a thin gold film. Nanocavities were incorporated into the sensor platform during the printing process and nanocones were incorporated during the 100 nm gold deposition process. Fluorescently-tagged single-strand (SS) DNA molecules were immobilized onto the surface and were designed such that the molecules would fluoresce when bound to a complementary sequence. Sensor substrates were imaged after exposure to a mismatched and matched oligomer to quantify the fluorescence enhancement of the sensor. Much higher fluorescence intensity was observed on all of the plasmonic structures as compared to flat gold.
AB - In this article, a novel plasmonic grating sensor platform was developed and tested for feasibility in sensor applications using a "lights-on" fluorescence based DNA sensor. The sensor platform combined the fluorescence enhancement of a grating-based plasmonic platform with the electric field intensifying effects of nano-scale cones and cavities. The gratings were made through a microcontact printing process that replicated HD-DVD discs in polymethylsilsesquioxane (PMSSQ) and coated in a thin gold film. Nanocavities were incorporated into the sensor platform during the printing process and nanocones were incorporated during the 100 nm gold deposition process. Fluorescently-tagged single-strand (SS) DNA molecules were immobilized onto the surface and were designed such that the molecules would fluoresce when bound to a complementary sequence. Sensor substrates were imaged after exposure to a mismatched and matched oligomer to quantify the fluorescence enhancement of the sensor. Much higher fluorescence intensity was observed on all of the plasmonic structures as compared to flat gold.
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U2 - 10.1109/ICSENS.2014.6985294
DO - 10.1109/ICSENS.2014.6985294
M3 - Article
AN - SCOPUS:84931051863
VL - 2014-December
SP - 1479
EP - 1482
JO - Proceedings of IEEE Sensors
JF - Proceedings of IEEE Sensors
SN - 1930-0395
IS - December
M1 - 6985294
ER -