Extracellular fluid conductivity analysis by dielectric spectroscopy for in vitro determination of cortical tissue vitality

K. F. Dobiszewski, M. P. Deek, A. Ghaly, C. Prodan, A. A. Hill

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Brain tissue is extremely metabolically active in part due to its need to constantly maintain a precise extracellular ionic environment. Under pathological conditions, unhealthy cortical tissue loses its ability to maintain this precise environment and there is a net efflux of charged particles from the cells. Typically, this ionic efflux is measured using ion-selective microelectrodes, which measure a single ionic species at a time. In this paper, we have used a bio-sensing method, dielectric spectroscopy (DS), which allows for the simultaneous measurement of the net efflux of all charged particles from cells by measuring extracellular conductivity. We exposed cortical brain slices from the mouse to different solutions that mimic various pathological states such as hypokalemia, hyperkalemia and ischemia (via oxygen-glucose deprivation). We have found that the changes in conductivity of the extracellular solutions were proportional to the severity of the pathological insult experienced by the brain tissue. Thus, DS allows for the measurement of changes in extracellular conductivity with enough sensitivity to monitor the health of brain tissue in vitro.

Original languageEnglish (US)
Pages (from-to)1249-1260
Number of pages12
JournalPhysiological Measurement
Volume33
Issue number7
DOIs
StatePublished - Jul 2012

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Physiology
  • Biomedical Engineering
  • Physiology (medical)

Keywords

  • dielectric spectroscopy
  • extracellular fluid conductivity
  • neural ion flux

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