Fluorescence activated cell sorting for enzymatic activity

Research output: Contribution to journalReview articlepeer-review

14 Scopus citations

Abstract

Directed evolution is a reliable method for protein engineering and as a tool for investigating structure/function relationships. A key for a successful directed evolution experiment is oftentimes the screen. Fluorescence activated cell sorting (FACS) is powerful high-throughput screening approach to isolate and identify mutants from large protein libraries. FACS has been successful in isolating proteins with improved or altered binding affinity. However, FACS screening for mutants with enhanced catalytic activity has been met with limited success. This review focuses on the FACS screening of protein libraries for enzymatic activity.

Original languageEnglish (US)
Pages (from-to)321-328
Number of pages8
JournalCombinatorial Chemistry and High Throughput Screening
Volume9
Issue number4
DOIs
StatePublished - May 1 2006

All Science Journal Classification (ASJC) codes

  • Chemistry (miscellaneous)
  • Clinical Biochemistry
  • Pharmacology

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