Folate receptor targeting silica nanoparticle probe for two-photon fluorescence bioimaging

Xuhua Wang, Sheng Yao, Hyo Yang Ahn, Yuanwei Zhang, Mykhailo V. Bondar, Joseph A. Torres, Kevin D. Belfield

Research output: Contribution to journalArticlepeer-review

38 Scopus citations


Narrow dispersity organically modified silica nanoparticles (SiNPs), diameter ~30 nm, entrapping a hydrophobic two-photon absorbing fluorenyl dye, were synthesized by hydrolysis of triethoxyvinylsilane and (3-aminopropyl)triethoxysilane in the nonpolar core of Aerosol-OT micelles. The surface of the SiNPs were functionalized with folic acid, to specifically deliver the probe to folate receptor (FR) over-expressing Hela cells, making these folate two-photon dye-doped SiNPs potential candidates as probes for two-photon fluorescence microscopy (2PFM) bioimaging. In vitro studies using FR over-expressing Hela cells and low FR expressing MG63 cells demonstrated specific cellular uptake of the functionalized nanoparticles. One-photon fluorescence microscopy (1PFM) imaging, 2PFM imaging, and two-photon fluorescence lifetime microscopy (2PFLIM) imaging of Hela cells incubated with folate-modified two-photon dye-doped SiNPs were demonstrated.

Original languageEnglish (US)
Pages (from-to)453-462
Number of pages10
JournalBiomedical Optics Express
Issue number2
StatePublished - Sep 1 2010
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Atomic and Molecular Physics, and Optics


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