Generation of folliculogenic human epithelial stem cells from induced pluripotent stem cells

Ruifeng Yang, Ying Zheng, Michelle Burrows, Shujing Liu, Zhi Wei, Arben Nace, Wei Guo, Suresh Kumar, George Cotsarelis, Xiaowei Xu

Research output: Contribution to journalArticlepeer-review

59 Scopus citations

Abstract

Epithelial stem cells (EpSCs) in the hair follicle bulge are required for hair follicle growth and cycling. The isolation and propagation of human EpSCs for tissue engineering purposes remains a challenge. Here we develop a strategy to differentiate human iPSCs (hiPSCs) into CD200 + /ITGA6 + EpSCs that can reconstitute the epithelial components of the hair follicle and interfollicular epidermis. The hiPSC-derived CD200 + /ITGA6 + cells show a similar gene expression signature as EpSCs directly isolated from human hair follicles. Human iPSC-derived CD200 + /ITGA6 + cells are capable of generating all hair follicle lineages including the hair shaft, and the inner and outer root sheaths in skin reconstitution assays. The regenerated hair follicles possess a KRT15 + stem cell population and produce hair shafts expressing hair-specific keratins. These results suggest an approach for generating large numbers of human EpSCs for tissue engineering and new treatments for hair loss, wound healing and other degenerative skin disorders.

Original languageEnglish (US)
Article number3071
JournalNature communications
Volume5
DOIs
StatePublished - Jan 28 2014

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Fingerprint Dive into the research topics of 'Generation of folliculogenic human epithelial stem cells from induced pluripotent stem cells'. Together they form a unique fingerprint.

Cite this