Identification and separation of DNA-hybridized nanocolloids by Taylor cone harmonics

Xinguang Cheng, Sagnik Basuray, Satyajyoti Senapati, Hsueh Chia Chang

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

A rapid (minutes) electrospray bead-based DNA hybridization detection technique is developed by spraying a mixture of hybridized and unhybridized silica nanocolloids. With proper far-field control by external electrodes, the trajectory of the ejected nanobeads from the electrospray is governed by specific harmonics of the Laplace equation, which select discrete polar angles along well-separated field maxima near the conducting Taylor cone. Due to Rayleigh fission and evaporation, beads of different size acquire different total charge after ejection and suffer different normal electrophoretic displacement such that they are ejected along well-separated field maxima and are deposited in distinct rings on an intersecting plane. As the hybridized DNA is of the same dimension as that of the nanocolloid, the nanocolloids are hence easily differentiated from the unhybridized ones. This technique is highly specific as the high shear stress in the microjet shears away any non-specifically bound DNA from the nanocolloid surface.

Original languageEnglish (US)
Pages (from-to)3236-3241
Number of pages6
JournalElectrophoresis
Volume30
Issue number18
DOIs
StatePublished - Sep 2009
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

Keywords

  • DNA
  • Electrospray
  • Harmonics
  • Hybridization
  • Nanocolloids

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