Imaging the bipolarity of myosin filaments with interferometric second Harmonic generation microscopy

Maxime Rivard; Charles André Couture; Amir K. Miri; Mathieu Laliberté; Antony Bertrand-Grenier; Luc Mongeau; François Légaré

doi:10.1364/BOE.4.002078

Imaging the bipolarity of myosin filaments with interferometric second Harmonic generation microscopy

Maxime Rivard, Charles André Couture, Amir K. Miri, Mathieu Laliberté, Antony Bertrand-Grenier, Luc Mongeau, François Légaré

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

We report that combining interferometry with Second Harmonic Generation (SHG) microscopy provides valuable information about the relative orientation of noncentrosymmetric structures composing tissues. This is confirmed through the imaging of rat medial gastrocnemius muscle. The inteferometric Second Harmonic Generation (ISHG) images reveal that each side of the myosin filaments composing the A band of the sarcomere generates π phase shifted SHG signal which implies that the myosin proteins at each end of the filaments are oriented in opposite directions. This highlights the bipolar structural organization of the myosin filaments and shows that muscles can be considered as a periodically poled biological structure.

Original language	English (US)
Pages (from-to)	2078-2086
Number of pages	9
Journal	Biomedical Optics Express
Volume	4
Issue number	10
DOIs	https://doi.org/10.1364/BOE.4.002078
State	Published - 2013
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biotechnology
Atomic and Molecular Physics, and Optics

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10.1364/BOE.4.002078

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abstract = "We report that combining interferometry with Second Harmonic Generation (SHG) microscopy provides valuable information about the relative orientation of noncentrosymmetric structures composing tissues. This is confirmed through the imaging of rat medial gastrocnemius muscle. The inteferometric Second Harmonic Generation (ISHG) images reveal that each side of the myosin filaments composing the A band of the sarcomere generates π phase shifted SHG signal which implies that the myosin proteins at each end of the filaments are oriented in opposite directions. This highlights the bipolar structural organization of the myosin filaments and shows that muscles can be considered as a periodically poled biological structure.",

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AU - Rivard, Maxime

AU - Couture, Charles André

AU - Miri, Amir K.

AU - Laliberté, Mathieu

AU - Bertrand-Grenier, Antony

AU - Mongeau, Luc

AU - Légaré, François

PY - 2013

Y1 - 2013

N2 - We report that combining interferometry with Second Harmonic Generation (SHG) microscopy provides valuable information about the relative orientation of noncentrosymmetric structures composing tissues. This is confirmed through the imaging of rat medial gastrocnemius muscle. The inteferometric Second Harmonic Generation (ISHG) images reveal that each side of the myosin filaments composing the A band of the sarcomere generates π phase shifted SHG signal which implies that the myosin proteins at each end of the filaments are oriented in opposite directions. This highlights the bipolar structural organization of the myosin filaments and shows that muscles can be considered as a periodically poled biological structure.

AB - We report that combining interferometry with Second Harmonic Generation (SHG) microscopy provides valuable information about the relative orientation of noncentrosymmetric structures composing tissues. This is confirmed through the imaging of rat medial gastrocnemius muscle. The inteferometric Second Harmonic Generation (ISHG) images reveal that each side of the myosin filaments composing the A band of the sarcomere generates π phase shifted SHG signal which implies that the myosin proteins at each end of the filaments are oriented in opposite directions. This highlights the bipolar structural organization of the myosin filaments and shows that muscles can be considered as a periodically poled biological structure.

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Imaging the bipolarity of myosin filaments with interferometric second Harmonic generation microscopy

Abstract

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