TY - GEN
T1 - Imaging the mechanisms of axon stretch growth
AU - Loverde, J. R.
AU - Ozoka, V. C.
AU - Aquino, R.
AU - Tolentino, R. T.
AU - Lin, L.
AU - Pfister, B. J.
PY - 2010
Y1 - 2010
N2 - The transition from embryo to adulthood involves a massive growth in the nervous system where axons in nerves extend and expand to accommodate to this growth. Applying mechanical forces to dorsal root ganglia neuronal cultures has previously shown that there is stimulation in axonal growth, gradually elongating the axon over a set period of time. In this research, the main focus is to study with live imaging how natural biomechanical forces, associated with growth of an organism, initiate unique neurobiological mechanisms that help drive the formation of long nerves. For this purpose, a bioreactor was developed for live imaging of stretch-growth as it occurs on the stage of a microscope. The bioreactor is independent from an incubator with external temperature controller and heating system regulated its physiological conditions. Morphology changes and cytoskeletal transport were captured live at magnifications up to 60x over weeks of culturing.
AB - The transition from embryo to adulthood involves a massive growth in the nervous system where axons in nerves extend and expand to accommodate to this growth. Applying mechanical forces to dorsal root ganglia neuronal cultures has previously shown that there is stimulation in axonal growth, gradually elongating the axon over a set period of time. In this research, the main focus is to study with live imaging how natural biomechanical forces, associated with growth of an organism, initiate unique neurobiological mechanisms that help drive the formation of long nerves. For this purpose, a bioreactor was developed for live imaging of stretch-growth as it occurs on the stage of a microscope. The bioreactor is independent from an incubator with external temperature controller and heating system regulated its physiological conditions. Morphology changes and cytoskeletal transport were captured live at magnifications up to 60x over weeks of culturing.
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U2 - 10.1109/NEBC.2010.5458137
DO - 10.1109/NEBC.2010.5458137
M3 - Conference contribution
AN - SCOPUS:77953072666
SN - 9781424468799
T3 - Proceedings of the 2010 IEEE 36th Annual Northeast Bioengineering Conference, NEBEC 2010
BT - Proceedings of the 2010 IEEE 36th Annual Northeast Bioengineering Conference, NEBEC 2010
T2 - 36th Annual Northeast Bioengineering Conference, NEBEC 2010
Y2 - 26 March 2010 through 28 March 2010
ER -