@article{cdb8f300a0c94d44a567a93a0645ab84,
title = "In vivo neuroprotective effect of a self-assembled peptide hydrogel",
abstract = "Traumatic brain injury (TBI) is associated with poor intrinsic healing responses and long-term cognitive decline. A major pathological outcome of TBI is acute glutamate-mediated excitotoxicity (GME) experienced by neurons. Short peptides based on the neuroprotective extracellular glycoprotein ependymin have shown the ability to slow down the effect of GME — however, such short peptides tend to diffuse away from target sites after in vivo delivery. We have designed a self-assembling peptide containing an ependymin mimic that can form nanofibrous matrices. The peptide was evaluated in situ to assess neuroprotective utility after an acute fluid-percussion injury. This biomimetic matrix can conform to the intracranial damaged site after delivery, due its shear-responsive rheological properties. We demonstrated the potential efficacy of the peptide for supporting neuronal survival in vitro and in vivo. Our study demonstrates the potential of these implantable acellular hydrogels for managing the acute (up to 7 days) pathophysiological sequelae after traumatic brain injury. Further work is needed to evaluate less invasive administrative routes and long-term functional and behavioral improvements after injury.",
keywords = "Ependymin, Neuroprotection, Self-assembly, Traumatic brain injury",
author = "Biplab Sarkar and Xiaotang Ma and Agnieszka Agas and Zain Siddiqui and Patricia Iglesias-Montoro and Nguyen, {Peter K.} and Kim, {Ka Kyung} and James Haorah and Kumar, {Vivek A.}",
note = "Funding Information: General : We would like to thank Dr. Roman Brukh (Rutgers) for characterization. We thank Dr. Volha Liaudanskaya and Dr. Jonathan Grasman for helpful discussions and feedback. Funding: This work was supported by grants NIH 1R21AA028340-01, GRANT12882559 (to J.H.) and NIH R15 EY029504, the NJIT Undergraduate Research and Innovation (URI) Program and NJIT Startup funds (to V.A.K.). Funding Information: General: We would like to thank Dr. Roman Brukh (Rutgers) for characterization. We thank Dr. Volha Liaudanskaya and Dr. Jonathan Grasman for helpful discussions and feedback. Funding: This work was supported by grants NIH 1R21AA028340-01, GRANT12882559 (to J.H.) and NIH R15 EY029504, the NJIT Undergraduate Research and Innovation (URI) Program and NJIT Startup funds (to V.A.K.). All procedures performed in studies involving animals followed the National Institutes of Health guidelines for the ethical care of laboratory animals, and the Institutional Animal Care and Use Committee (IACUC) at the animal facility of Rutgers University (Newark). B.S. and X.M. contributed equally. Experiments and data acquisitions were performed by B.S. Z.S. K.K, P.K.N, P.I.M. A.A. and X.M. The manuscript was written by B.S. and X.M. J.H. and V.A.K. supervised the development of experiments, provided critical feedback, and edited the manuscript. All authors have given approval to the final version of the manuscript. Additional data related to this paper may be requested from the authors. Publisher Copyright: {\textcopyright} 2020 Elsevier B.V.",
year = "2021",
month = mar,
day = "15",
doi = "10.1016/j.cej.2020.127295",
language = "English (US)",
volume = "408",
journal = "Chemical Engineering Journal",
issn = "1385-8947",
publisher = "Elsevier",
}