Indentation of poroviscoelastic vocal fold tissue using an atomic force microscope

Hossein K. Heris, Amir K. Miri, Umakanta Tripathy, Francois Barthelat, Luc Mongeau

Research output: Contribution to journalArticlepeer-review

28 Scopus citations


The elastic properties of the vocal folds (VFs) vary as a function of depth relative to the epithelial surface. The poroelastic anisotropic properties of porcine VFs, at various depths, were measured using atomic force microscopy (AFM)-based indentation. The minimum tip diameter to effectively capture the local properties was found to be 25μm, based on nonlinear laser scanning microscopy data and image analysis. The effects of AFM tip dimensions and AFM cantilever stiffness were systematically investigated. The indentation tests were performed along the sagittal and coronal planes for an evaluation of the VF anisotropy. Hertzian contact theory was used along with the governing equations of linear poroelasticity to calculate the diffusivity coefficient of the tissue from AFM indentation creep testing. The permeability coefficient of the porcine VF was found to be 1.80±0.32×10-15m4/Ns.

Original languageEnglish (US)
Pages (from-to)383-392
Number of pages10
JournalJournal of the Mechanical Behavior of Biomedical Materials
StatePublished - Dec 2013
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biomaterials
  • Biomedical Engineering
  • Mechanics of Materials


  • Anisotropy
  • Atomic force microscopy
  • Indentation
  • Nonlinear optics
  • Permeability
  • Vocal folds


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