Indentation of poroviscoelastic vocal fold tissue using an atomic force microscope

Hossein K. Heris; Amir K. Miri; Umakanta Tripathy; Francois Barthelat; Luc Mongeau

doi:10.1016/j.jmbbm.2013.05.026

Indentation of poroviscoelastic vocal fold tissue using an atomic force microscope

Hossein K. Heris, Amir K. Miri, Umakanta Tripathy, Francois Barthelat, Luc Mongeau

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

The elastic properties of the vocal folds (VFs) vary as a function of depth relative to the epithelial surface. The poroelastic anisotropic properties of porcine VFs, at various depths, were measured using atomic force microscopy (AFM)-based indentation. The minimum tip diameter to effectively capture the local properties was found to be 25μm, based on nonlinear laser scanning microscopy data and image analysis. The effects of AFM tip dimensions and AFM cantilever stiffness were systematically investigated. The indentation tests were performed along the sagittal and coronal planes for an evaluation of the VF anisotropy. Hertzian contact theory was used along with the governing equations of linear poroelasticity to calculate the diffusivity coefficient of the tissue from AFM indentation creep testing. The permeability coefficient of the porcine VF was found to be 1.80±0.32×10^-15m⁴/Ns.

Original language	English (US)
Pages (from-to)	383-392
Number of pages	10
Journal	Journal of the Mechanical Behavior of Biomedical Materials
Volume	28
DOIs	https://doi.org/10.1016/j.jmbbm.2013.05.026
State	Published - Dec 2013
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biomaterials
Biomedical Engineering
Mechanics of Materials

Keywords

Anisotropy
Atomic force microscopy
Indentation
Nonlinear optics
Permeability
Vocal folds

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10.1016/j.jmbbm.2013.05.026

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title = "Indentation of poroviscoelastic vocal fold tissue using an atomic force microscope",

abstract = "The elastic properties of the vocal folds (VFs) vary as a function of depth relative to the epithelial surface. The poroelastic anisotropic properties of porcine VFs, at various depths, were measured using atomic force microscopy (AFM)-based indentation. The minimum tip diameter to effectively capture the local properties was found to be 25μm, based on nonlinear laser scanning microscopy data and image analysis. The effects of AFM tip dimensions and AFM cantilever stiffness were systematically investigated. The indentation tests were performed along the sagittal and coronal planes for an evaluation of the VF anisotropy. Hertzian contact theory was used along with the governing equations of linear poroelasticity to calculate the diffusivity coefficient of the tissue from AFM indentation creep testing. The permeability coefficient of the porcine VF was found to be 1.80±0.32×10-15m4/Ns.",

keywords = "Anisotropy, Atomic force microscopy, Indentation, Nonlinear optics, Permeability, Vocal folds",

author = "Heris, {Hossein K.} and Miri, {Amir K.} and Umakanta Tripathy and Francois Barthelat and Luc Mongeau",

year = "2013",

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doi = "10.1016/j.jmbbm.2013.05.026",

language = "English (US)",

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AU - Heris, Hossein K.

AU - Miri, Amir K.

AU - Tripathy, Umakanta

AU - Barthelat, Francois

AU - Mongeau, Luc

PY - 2013/12

Y1 - 2013/12

N2 - The elastic properties of the vocal folds (VFs) vary as a function of depth relative to the epithelial surface. The poroelastic anisotropic properties of porcine VFs, at various depths, were measured using atomic force microscopy (AFM)-based indentation. The minimum tip diameter to effectively capture the local properties was found to be 25μm, based on nonlinear laser scanning microscopy data and image analysis. The effects of AFM tip dimensions and AFM cantilever stiffness were systematically investigated. The indentation tests were performed along the sagittal and coronal planes for an evaluation of the VF anisotropy. Hertzian contact theory was used along with the governing equations of linear poroelasticity to calculate the diffusivity coefficient of the tissue from AFM indentation creep testing. The permeability coefficient of the porcine VF was found to be 1.80±0.32×10-15m4/Ns.

AB - The elastic properties of the vocal folds (VFs) vary as a function of depth relative to the epithelial surface. The poroelastic anisotropic properties of porcine VFs, at various depths, were measured using atomic force microscopy (AFM)-based indentation. The minimum tip diameter to effectively capture the local properties was found to be 25μm, based on nonlinear laser scanning microscopy data and image analysis. The effects of AFM tip dimensions and AFM cantilever stiffness were systematically investigated. The indentation tests were performed along the sagittal and coronal planes for an evaluation of the VF anisotropy. Hertzian contact theory was used along with the governing equations of linear poroelasticity to calculate the diffusivity coefficient of the tissue from AFM indentation creep testing. The permeability coefficient of the porcine VF was found to be 1.80±0.32×10-15m4/Ns.

KW - Anisotropy

KW - Atomic force microscopy

KW - Indentation

KW - Nonlinear optics

KW - Permeability

KW - Vocal folds

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JO - Journal of the Mechanical Behavior of Biomedical Materials

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ER -

Indentation of poroviscoelastic vocal fold tissue using an atomic force microscope

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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