Abstract
Infrared (vibrational) circular dichroism (VCD) spectra have been obtained for the self-complementary tetranucleotides, 5'd(CGCG)3', 5'd(GCGC)3', 5'd(CCGG)3', and 5'd(GGCC)3'. In buffered aqueous solution at low salt concentration, these tetramers exhibit spectra associated with right-handed polymers, although the spectra differ significantly for the four species. In high salt solution, a B-->Z transition occurs in 5'd(CGCG)3', while the other tetranucleotides appear only slightly altered. Temperature dependent studies of these oligonucleotides reveal a greater amount of thermal stability for the tetramers in low salt than for the high salt solutions. VCD intensities computed via the exciton formalism are compared with observed results.
Original language | English (US) |
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Pages (from-to) | 1262-1271 |
Number of pages | 10 |
Journal | Biophysical Journal |
Volume | 65 |
Issue number | 3 |
DOIs | |
State | Published - 1993 |
Externally published | Yes |
All Science Journal Classification (ASJC) codes
- Biophysics