TY - JOUR
T1 - Insights into the roles of biochar pores toward alleviating antibiotic resistance genes accumulation in biofiltration systems
AU - Wei, Lecheng
AU - Zheng, Jingjing
AU - Han, Yutong
AU - Xu, Xiangyang
AU - Li, Mengyan
AU - Zhu, Liang
N1 - Publisher Copyright:
© 2023 Elsevier Ltd
PY - 2024/2
Y1 - 2024/2
N2 - Biofiltration systems would harbor and spread various antibiotic resistance genes (ARGs) when treating antibiotic micro-pollution, constituting a potential ecological risk. This study aimed to investigate the effects of biochar pores on ARG emergence and related microbial response mechanisms in bench-scale biofiltration systems. Results showed that biochar pores effectively reduced the absolute copies of the corresponding ARGs sul1 and sul2 by 54.1% by lowering the sorbed-SMX's bioavailability compared to non-porous anthracite. An investigation of antimicrobial resistomes revealed a considerable decrease in the abundance and diversity of ARGs and mobile gene elements. Metagenomic and metaproteomic analysis demonstrated that biochar pores induced the changeover of microbial defense strategy against SMX from blocking SMX uptake by EPS absorbing to SMX biotransformation. Microbial SOS response, antibiotic efflux pump, EPS secretion, and biofilm formation were decreased. Functions related to SMX biotransformation, such as sadABC-mediated transformation, xenobiotics degradation, and metabolism, were significantly promoted.
AB - Biofiltration systems would harbor and spread various antibiotic resistance genes (ARGs) when treating antibiotic micro-pollution, constituting a potential ecological risk. This study aimed to investigate the effects of biochar pores on ARG emergence and related microbial response mechanisms in bench-scale biofiltration systems. Results showed that biochar pores effectively reduced the absolute copies of the corresponding ARGs sul1 and sul2 by 54.1% by lowering the sorbed-SMX's bioavailability compared to non-porous anthracite. An investigation of antimicrobial resistomes revealed a considerable decrease in the abundance and diversity of ARGs and mobile gene elements. Metagenomic and metaproteomic analysis demonstrated that biochar pores induced the changeover of microbial defense strategy against SMX from blocking SMX uptake by EPS absorbing to SMX biotransformation. Microbial SOS response, antibiotic efflux pump, EPS secretion, and biofilm formation were decreased. Functions related to SMX biotransformation, such as sadABC-mediated transformation, xenobiotics degradation, and metabolism, were significantly promoted.
KW - ARGs
KW - Biochar
KW - Biofiltration system
KW - Microbial metabolism
KW - Pore
UR - http://www.scopus.com/inward/record.url?scp=85181815872&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85181815872&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2023.130257
DO - 10.1016/j.biortech.2023.130257
M3 - Article
C2 - 38151208
AN - SCOPUS:85181815872
SN - 0960-8524
VL - 394
JO - Bioresource Technology
JF - Bioresource Technology
M1 - 130257
ER -