Integrated capillary fluorescence DNA biosensor

Marc A. Breimer, Yevgeny Gelfand, Omowunmi A. Sadik

Research output: Contribution to journalArticlepeer-review

24 Scopus citations


Covalent attachment of dsDNA molecules inside a glass capillary without the need for hybridization is described. It is shown that the glass capillary has a surface density of 2.5×1013 molecules/cm2 with specific binding capacity of 62.5%. The resulting substrate was used to develop a biosensor for determining fluorescent organic analytes and metal binding with DNA. The biosensor combines highly specific immobilization chemistry with a capillary-geometry flow cell arrangement. The results show that fluorescent dyes are retained in the dsDNA-modified surface and that exposure to concentrations of nickel and lead ions resulted in a recoverable, highly reproducible diminishment of the fluorescence intensity.

Original languageEnglish (US)
Pages (from-to)1135-1147
Number of pages13
JournalBiosensors and Bioelectronics
Issue number9
StatePublished - Aug 15 2003
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry


  • Biosensor
  • Capillary fluorescence
  • DNA


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