Integrating waveguide biosensor

Frances S. Ligler; Marc Breimer; Joel P. Golden; Delana A. Nivens; James P. Dodson; Tiffanee M. Green; Daniel P. Haders; Omowunmi A. Sadik

doi:10.1021/ac015607s

Integrating waveguide biosensor

Frances S. Ligler, Marc Breimer, Joel P. Golden, Delana A. Nivens, James P. Dodson, Tiffanee M. Green, Daniel P. Haders, Omowunmi A. Sadik

Research output: Contribution to journal › Article › peer-review

97 Scopus citations

Abstract

A capillary biosensor is demonstrated which uses the waveguiding properties of the capillary to integrate the signal over an increased surface area without simultaneously increasing the background noise from the detector. This biosensor achieves limits of detection of 30-50 pg/mL in immunoassays using a diode laser for excitation and a PMT for detection. This is ∼2 orders of magnitude greater sensitivity than was achieved using the same immunoassay reagents in a fiber optic biosensor or a planar array biosensor. Two different approaches to using the capillaries as immunosensors are described, either of which could be adapted for multianalyte sensing.

Original language	English (US)
Pages (from-to)	713-719
Number of pages	7
Journal	Analytical Chemistry
Volume	74
Issue number	3
DOIs	https://doi.org/10.1021/ac015607s
State	Published - Feb 1 2002
Externally published	Yes

All Science Journal Classification (ASJC) codes

Analytical Chemistry

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10.1021/ac015607s

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title = "Integrating waveguide biosensor",

abstract = "A capillary biosensor is demonstrated which uses the waveguiding properties of the capillary to integrate the signal over an increased surface area without simultaneously increasing the background noise from the detector. This biosensor achieves limits of detection of 30-50 pg/mL in immunoassays using a diode laser for excitation and a PMT for detection. This is ∼2 orders of magnitude greater sensitivity than was achieved using the same immunoassay reagents in a fiber optic biosensor or a planar array biosensor. Two different approaches to using the capillaries as immunosensors are described, either of which could be adapted for multianalyte sensing.",

author = "Ligler, {Frances S.} and Marc Breimer and Golden, {Joel P.} and Nivens, {Delana A.} and Dodson, {James P.} and Green, {Tiffanee M.} and Haders, {Daniel P.} and Sadik, {Omowunmi A.}",

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T1 - Integrating waveguide biosensor

AU - Ligler, Frances S.

AU - Breimer, Marc

AU - Golden, Joel P.

AU - Nivens, Delana A.

AU - Dodson, James P.

AU - Green, Tiffanee M.

AU - Haders, Daniel P.

AU - Sadik, Omowunmi A.

PY - 2002/2/1

Y1 - 2002/2/1

N2 - A capillary biosensor is demonstrated which uses the waveguiding properties of the capillary to integrate the signal over an increased surface area without simultaneously increasing the background noise from the detector. This biosensor achieves limits of detection of 30-50 pg/mL in immunoassays using a diode laser for excitation and a PMT for detection. This is ∼2 orders of magnitude greater sensitivity than was achieved using the same immunoassay reagents in a fiber optic biosensor or a planar array biosensor. Two different approaches to using the capillaries as immunosensors are described, either of which could be adapted for multianalyte sensing.

AB - A capillary biosensor is demonstrated which uses the waveguiding properties of the capillary to integrate the signal over an increased surface area without simultaneously increasing the background noise from the detector. This biosensor achieves limits of detection of 30-50 pg/mL in immunoassays using a diode laser for excitation and a PMT for detection. This is ∼2 orders of magnitude greater sensitivity than was achieved using the same immunoassay reagents in a fiber optic biosensor or a planar array biosensor. Two different approaches to using the capillaries as immunosensors are described, either of which could be adapted for multianalyte sensing.

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VL - 74

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JO - Analytical Chemistry

JF - Analytical Chemistry

IS - 3

ER -

Integrating waveguide biosensor

Abstract

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