Luminescent probes for ultrasensitive detection of nucleic acids

Lev N. Krasnoperov, Salvatore A.E. Marras, Maxim Kozlov, Laura Wirpsza, Arkady Mustaev

Research output: Contribution to journalArticlepeer-review

44 Scopus citations

Abstract

Novel amino-reactive derivatives of lanthanide-based luminescent labels of enhanced brightness and metal retention were synthesized and used for the detection of cDNA oligonucleotides by molecular beacons. Time-resolved acquisition of the luminescent signal that occurs upon hybridization of the probe to the target enabled the avoidance of short-lived background fluorescence, markedly enhancing the sensitivity of detection, which was less than 1 pM. This value is about 50 to 100 times more sensitive than the level achieved with conventional fluorescencebased molecular beacons, and is 10 to 60 times more sensitive than previously reported for other lanthanidebased hybridization probes. These novel luminescent labels should significantly enhance the sensitivity of all type of nucleic acid hybridization probes, and could dramatically improve the detection limit of other biopolymers and small compounds that are used in a variety of biological applications.

Original languageEnglish (US)
Pages (from-to)319-327
Number of pages9
JournalBioconjugate Chemistry
Volume21
Issue number2
DOIs
StatePublished - Feb 17 2010

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry

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