Mapping the functional connectivity of anterior cingulate cortex

Daniel S. Margulies, A. M.Clare Kelly, Lucina Q. Uddin, Bharat B. Biswal, F. Xavier Castellanos, Michael P. Milham

Research output: Contribution to journalArticlepeer-review

618 Scopus citations


Anterior cingulate cortex (ACC) is a nexus of information processing and regulation in the brain. Reflecting this central role, ACC is structurally and functionally heterogeneous, a fact long appreciated in studies of non-human primates. Human neuroimaging studies also recognize this functional heterogeneity, with meta-analyses and task-based studies demonstrating the existence of motor, cognitive and affective subdivisions. In contrast to task-based approaches, examinations of resting-state functional connectivity enable the characterization of task-independent patterns of correlated activity. In a novel approach to understanding ACC functional segregation, we systematically mapped ACC functional connectivity during rest. We examined patterns of functional connectivity for 16 seed ROIs systematically placed throughout caudal, rostral, and subgenual ACC in each hemisphere. First, our data support the commonly observed rostral/caudal distinction, but also suggest the existence of a dorsal/ventral functional distinction. For each of these distinctions, more fine-grained patterns of differentiation were observed than commonly appreciated in human imaging studies. Second, we demonstrate the presence of negatively predicted relationships between distinct ACC functional networks. In particular, we highlight negative relationships between rostral ACC-based affective networks (including the "default mode network") and dorsal-caudal ACC-based frontoparietal attention networks. Finally, interhemispheric activations were more strongly correlated between homologous regions than in non-homologous regions. We discuss the implications of our work for understanding ACC function and potential applications to clinical populations.

Original languageEnglish (US)
Pages (from-to)579-588
Number of pages10
Issue number2
StatePublished - Aug 15 2007
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Neurology
  • Cognitive Neuroscience


  • Anticorrelation
  • Brain mapping
  • Intrinsic functional activity
  • Resting state networks


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