Micro-fluidic channels on nanopatterned substrates: Monitoring protein binding to lipid bilayers with surface-enhanced Raman spectroscopy

Amrita Banerjee, R. Perez-Castillejos, D. Hahn, Alex I. Smirnov, H. Grebel

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

We used surface-enhanced Raman spectroscopy (SERS) to detect binding events between streptavidin and biotinylated lipid bilayers. The binding events took place at the surface between micro-fluidic channels and anodized aluminum oxide (AAO) with the latter serving as substrates. The bilayers were incorporated in the substrate pores. It was revealed that non-bound molecules were easily washed away and that large suspended cells (Salmonella enterica) are less likely to interfere with the monitoring process: when focusing to the lower surface of the channel, one may resolve mostly the bound molecules.

Original languageEnglish (US)
Pages (from-to)121-126
Number of pages6
JournalChemical Physics Letters
Volume489
Issue number1-3
DOIs
StatePublished - Apr 1 2010

All Science Journal Classification (ASJC) codes

  • Physics and Astronomy(all)
  • Physical and Theoretical Chemistry

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