Micro-scale membrane extraction of glyphosate and aminomethylphosphonic acid in water followed by high-performance liquid chromatography and post-column derivatization with fluorescence detector

Montra Piriyapittaya, Siripastr Jayanta, Somenath Mitra, Natchanun Leepipatpiboon

Research output: Contribution to journalArticlepeer-review

45 Scopus citations

Abstract

A carrier-mediated supported liquid membrane micro-extraction using single hollow fiber membrane suitable for the determination of the herbicide glyphosate and its main metabolite aminomethylphosphonic acid in water is reported. A solution of 0.20 M Aliquat-336, a cationic carrier, in di-n-hexyl ether was selected as the supported liquid. A 20 μL of 1.0 M potassium chloride as the acceptor phase was filled in the membrane lumen. The membrane was immersed in a 20 mL of pH 9.0 sample solution. After 60-min extraction, the acceptor phase was analyzed by high-performance liquid chromatography with post-column derivatization. The enrichment factors were found to be 853 and 136 for glyphosate and aminomethylphosphonic acid, respectively. The method detection limits are 0.22 μg/L for glyphosate and 3.40 μg/L for aminomethylphosphonic acid. The procedure was validated and showed good accuracy and precision over a large linear dynamic range. The validated method was tested for the analysis of both analytes in spiked groundwater with good success.

Original languageEnglish (US)
Pages (from-to)483-492
Number of pages10
JournalJournal of Chromatography A
Volume1189
Issue number1-2
DOIs
StatePublished - May 2 2008

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

Keywords

  • Aminomethylphosphonic acid
  • Carrier-mediated transport
  • Glyphosate
  • Membrane extraction
  • Micro-extraction
  • SLME

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