Microbiome signatures in prostate cancer

Sagarika Banerjee, James C. Alwine, Zhi Wei, Tian Tian, Natalie Shih, Colin Sperling, Thomas Guzzo, Michael D. Feldman, Erle S. Robertson

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

We have established a microbiome signature for prostate cancer using an array-based metagenomic and capture-sequencing approach. A diverse microbiome signature (viral, bacterial, fungal and parasitic) was observed in the prostate cancer samples compared with benign prostate hyperplasia controls. Hierarchical clustering analysis identified three distinct prostate cancer-specific microbiome signatures. The three signatures correlated with different grades, stages and scores of the cancer. Thus, microbiome signature analysis potentially provides clinical diagnosis and outcome predictions. The array data were validated by PCR and targeted next-generation sequencing (NGS). Specific NGS data suggested that certain viral genomic sequences were inserted into the host somatic chromosomes of the prostate cancer samples. A randomly selected group of these was validated by direct PCR and sequencing. In addition, PCR validation of Helicobacter showed that Helicobacter cagA sequences integrated within specific chromosomes of prostate tumor cells. The viral and Helicobacter integrations are predicted to affect the expression of several cellular genes associated with oncogenic processes.

Original languageEnglish (US)
Pages (from-to)749-764
Number of pages16
JournalCarcinogenesis
Volume40
Issue number6
DOIs
StatePublished - Jul 6 2019

All Science Journal Classification (ASJC) codes

  • Cancer Research

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